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首页> 外文期刊>Journal of Neuroscience Methods >Terminal effects of optogenetic stimulation on dopamine dynamics in rat striatum
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Terminal effects of optogenetic stimulation on dopamine dynamics in rat striatum

机译：遗传刺激对大鼠纹状体多巴胺动力学的最终影响

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In this study, the first in-depth analysis of optically induced dopamine release using fast-scan cyclic voltammetry on striatal slices from rat brain was performed. An adeno-associated virus that expresses Channelrhodopsin-2 was injected in the substantia nigra. Tissue was collected and sectioned into 400 ??m-thick coronal slices 4 weeks later. Blue laser light (473. nm) was delivered through a fiber optic inserted into slice tissue. Experiments revealed some difference between maximal amplitudes measured from optically and electrically evoked dopamine effluxes. Specifically, there was an increase in the amplitude of dopamine release induced by electrical stimulation in comparison with light stimulations. However, we found that dopamine release is more sensitive to changes in the pulse width in the case of optical stimulation. Light-stimulated dopamine was increased as the stimulation pulse widened. There was no difference with repeated stimulations at five minute intervals between stimulation sources and dopamine signal was stable during recording sessions, while one minute intervals resulted in a decline in the amplitude from both sources. Optical stimulation can also produce an artifact that is distinguishable from dopamine by the cyclic voltammogram. These results confirm that optical stimulation of dopamine is a sound approach for future pharmacological studies in slices. ? 2013 Elsevier B.V.
机译：在这项研究中，使用快速扫描循环伏安法对来自大鼠脑的纹状体切片进行了光学诱导的多巴胺释放的首次深入分析。将表达Channelrhodopsin-2的腺相关病毒注射到黑质中。 4周后，收集组织并切成400μm厚的冠状切片。蓝色激光（473. nm）通过插入切片组织的光纤传输。实验表明，从光学和电诱发的多巴胺流出量测得的最大振幅之间存在一些差异。具体而言，与光刺激相比，电刺激引起的多巴胺释放幅度增加。但是，我们发现在光刺激的情况下，多巴胺释放对脉冲宽度的变化更为敏感。随着刺激脉冲变宽，光刺激的多巴胺增加。刺激源之间以五分钟间隔重复刺激没有差异，并且在记录期间多巴胺信号稳定，而一分钟间隔导致两个源的振幅下降。光学刺激还可产生伪影，该伪影可通过循环伏安图与多巴胺区分开。这些结果证实，多巴胺的光刺激是将来在切片中进行药理研究的可靠方法。 ？ 2013年Elsevier B.V.

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