首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Pressure dependence of the apparent specific volume of bovine serum albumin: Insight into the difference between isothermal and adiabatic compressibilities.
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Pressure dependence of the apparent specific volume of bovine serum albumin: Insight into the difference between isothermal and adiabatic compressibilities.

机译:牛血清白蛋白表观比容的压力依赖性:了解等温和绝热可压缩性之间的差异。

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摘要

There are some theoretical arguments related to interpreting the adiabatic compressibility (beta(s)) of a protein determined from the sound velocity and the difference between beta(s) and isothermal compressibility (beta(T)). To address these problems experimentally, we constructed a high-pressure oscillating densitometer and used it to measure the apparent specific volume of bovine serum albumin as a function of pressure (0.1-78MPa) and temperature (5-35 degrees C). The beta(T) determined from plots of the apparent specific volume vs. pressure was slightly larger than beta(s) at all temperatures examined, with the difference between the two compressibilities increasing as the temperature was decreased. Only at room temperature did the observed beta(T) agree with those estimated from beta(s) using the heat capacity and the thermal expansibility of the protein, suggesting that there are significant as-yet-unknown mechanisms that affect protein compressibility.
机译:有一些理论上的论据与解释蛋白质的绝热可压缩性(β)有关,该绝热可压缩性由声速以及贝塔和等温压缩性(β(T))之间的差异确定。为了通过实验解决这些问题,我们构建了一个高压振荡光密度计,并用它来测量牛血清白蛋白的表观比容与压力(0.1-78MPa)和温度(5-35摄氏度)的关系。根据表观比容与压力的关系图确定的beta(T)在所有检查的温度下均比beta(s)稍大,并且随着温度降低,两种压缩率之间的差异也随之增加。仅在室温下,观察到的β(T)才与使用蛋白质的热容量和热膨胀性从β(s)估算的β(T)相符,这表明仍存在影响蛋白质可压缩性的重要机制。

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