• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Conformational stabilities of guinea pig OCP1 and OCP2.
【24h】

Conformational stabilities of guinea pig OCP1 and OCP2.

机译：豚鼠OCP1和OCP2的构象稳定性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

OCP1 and OCP2, the most abundant proteins in the cochlea, are evidently subunits of an SCF E3 ubiquitin ligase. Although transcribed from a distinct gene, OCP2 is identical to Skp1. OCP1 is equivalent to the F-box protein known as Fbs1, Fbx2, or NFB42 - previously shown to bind N-glycosylated proteins and believed to function in the retrieval and recycling of misfolded proteins. The high concentrations of OCP1 and OCP2 in the cochlea suggest that the OCP1-OCP2 heterodimer may serve an additional function independent of its role in a canonical SCF complex. At 25 degrees C, urea-induced denaturation of OCP1 is slow, but reversible. The data suggest that the protein possesses one or more disordered regions, a conclusion supported by analysis of the far-UV circular dichroism spectrum and the appearance of the (1)H, (15)N-HSQC spectrum. Thermal denaturation of OCP1 is irreversible, evidently due to formation of high molecular weight aggregates. Analysis with a kinetic model yields an estimate for the activation energy for unfolding of 49 kcal/mol. Urea denaturation data for OCP2 returns DeltaG(o) and m values of 6.2 kcal/mol and 1.5 kcal mol(-)(1) M(-1), respectively. In contrast to OCP1, thermal denaturation of OCP2 is reversible. In phosphate-buffered saline, at pH 7.40, the protein exhibits a DeltaH(vH)/DeltaH(cal) ratio of 1.69, suggesting that denaturation proceeds largely from the native dimer directly to the unfolded state. OCP1 and OCP2 associate tightly at room temperature. However, DSC data for the complex suggest that they denature independently, consistent with the highly exothermic enthalpy of complex formation reported previously.
机译：OCP1和OCP2是耳蜗中最丰富的蛋白质，显然是SCF E3泛素连接酶的亚基。尽管从不同的基因转录，OCP2与Skp1相同。 OCP1相当于F-box蛋白，称为Fbs1，Fbx2或NFB42-先前显示可结合N-糖基化蛋白，并被认为在错误折叠蛋白的回收和再循环中起作用。耳蜗中OCP1和OCP2的高浓度表明，OCP1-OCP2异二聚体可能具有其他功能，而与它在规范SCF复合物中的作用无关。在25摄氏度时，尿素诱导的OCP1变性很慢，但可逆。数据表明该蛋白质具有一个或多个无序区，这一结论得到了远紫外圆二色性光谱和（1）H，（15）N-HSQC光谱分析的支持。 OCP1的热变性是不可逆的，这显然是由于形成了高分子量聚集体。用动力学模型进行分析得出的估计活化能为49 kcal / mol。 OCP2的尿素变性数据分别返回DeltaG（o）和m值分别为6.2 kcal / mol和1.5 kcal mol（-）（1）M（-1）。与OCP1相比，OCP2的热变性是可逆的。在pH值为7.40的磷酸盐缓冲盐水中，蛋白质的DeltaH（vH）/ DeltaH（cal）比为1.69，这表明变性主要是从天然二聚体直接进行到未折叠状态。 OCP1和OCP2在室温下紧密结合。然而，该复合物的DSC数据表明它们独立地变性，这与先前报道的复合物形成的高放热焓一致。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号