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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Nucleic acid structure and sequence probing using fluorescent base analogue tC(O).
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Nucleic acid structure and sequence probing using fluorescent base analogue tC(O).

机译：使用荧光碱基类似物tC（O）进行核酸结构和序列探测。

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摘要

The fluorescent cytosine analog tC(O) is on average the brightest probe of its kind and, moreover, it introduces minimal perturbations to the normal secondary structure of DNA. Here several ways of how tC(O), with an advantage, can be used as a local fluorescent probe in nucleic acid systems are presented. Most importantly, we show that tC(O) is an excellent probe for the detection of individual melting processes of complex nucleic acid structures containing a large number of separate secondary structure motifs. Since conventional UV-melting investigations merely monitor the global melting process of the whole nucleic acid structure, e.g. multi-hairpin systems in RNA/DNA, and thus is incapable of estimating individual melting transitions of such systems, tC(O) represents a new method of characterization. Furthermore, we find that tC(O) may be used to detect bulges and loops in nucleic acids as well as to distinguish a matched base-pair from several of the mismatched.
机译：荧光胞嘧啶类似物tC（O）平均是同类探针中最亮的，而且，它对DNA的正常二级结构的干扰很小。此处介绍了如何将tC（O）用作核酸系统中的局部荧光探针的几种方法。最重要的是，我们表明tC（O）是用于检测包含大量单独二级结构基序的复杂核酸结构的单个解链过程的出色探针。由于常规的UV熔解研究仅监测整个核酸结构的整体熔解过程，例如tC（O）代表RNA / DNA中的多发夹系统，因此无法估算此类系统的单个解链转变，因此tC（O）代表了一种新的表征方法。此外，我们发现tC（O）可以用于检测核酸中的凸起和环，以及区分匹配的碱基对和一些错配的碱基。

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