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首页> 外文期刊>Journal of optics, A. Pure and applied optics: journal of the European Optical Society >Detection of the specific binding on protein microarrays by oblique-incidence reflectivity difference method
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Detection of the specific binding on protein microarrays by oblique-incidence reflectivity difference method

机译：斜入射反射率差法检测蛋白质微阵列上的特异性结合

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摘要

The specific binding between Cy5-labeled goat anti-mouse Immunoglobulin G (IgG) and mouse IgG with a concentration range from 625 to 10~4 μg ml~(-1) has been detected successfully by the oblique-incidence reflectivity difference (OI-RD) method in each procedure of microarray fabrication. The experimental data prove that the OI-RD method can be employed not only to distinguish the different concentrations in label-free fashion but also to detect the antibody–antigen capture. In addition, the differential treatment of the OI-RD signals can decrease the negative influences of glass slide as the microarray upholder. Therefore the OI-RD technique has promising applications for the label-free and high-throughput detection of protein microarrays.
机译：通过斜入射反射率差异（OI-）成功检测到Cy5标记的山羊抗小鼠免疫球蛋白G（IgG）与浓度在625至10〜4μgml〜（-1）的小鼠IgG之间的特异性结合。 RD）方法在微阵列制造的每个过程中。实验数据证明，OI-RD方法不仅可以用来以无标记的方式区分不同的浓度，而且可以检测抗体-抗原的捕获。此外，OI-RD信号的差异处理可以减少载玻片作为微阵列支持者的负面影响。因此，OI-RD技术在蛋白质微阵列的无标记和高通量检测方面具有广阔的应用前景。

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