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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Time course of the age-related alterations in stored blood
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Time course of the age-related alterations in stored blood

机译：血液中与年龄有关的变化的时程

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The extent and time course of the impairments occurring in whole blood and erythrocyte cells stored under blood bank conditions were studied by monitoring the reduction of MAL-6 spin label added to the media containing whole blood or erythrocyte cells using electron spin resonance (ESR) technique. Impairments forming in the erythrocyte cells incubated for various times at 37degreesC were also studied. Erythrocyte cells were found to undergo changes during the storage or incubation, leading to fast decay of MAL-6 spin labels signal height. The extent of the changes depends on storage or incubation time. However, the reduction in incubated or artificially aged erythrocyte (AAE) cells was faster than the reduction in whole blood (WB) and aged erythrocyte (AE) cells stored under blood bank conditions. Two exponential curves attributed to the liquid and cellular parts of a given samples were found to be described best in the reduction of MAL-6 spin label in WB, AE and AAE. (C) 2003 Elsevier B.V. All rights reserved. [References: 35]
机译：通过使用电子自旋共振（ESR）技术监测添加到含有全血或红细胞的培养基中的MAL-6旋转标记的减少，研究了在血库条件下存储的全血和红细胞中发生损伤的程度和时间过程。还研究了在37°C孵育不同时间的红细胞中形成的损伤。发现红细胞在储存或温育过程中发生变化，导致MAL-6自旋标记信号高度快速衰减。变化的程度取决于存储或孵育时间。但是，孵育或人工老化的红细胞（AAE）细胞的减少要快于在血库条件下存储的全血（WB）和老化的红细胞（AE）细胞的减少。发现归因于给定样品的液体和细胞部分的两条指数曲线在减少WB，AE和AAE中MAL-6自旋标记物方面得到了最好的描述。 （C）2003 Elsevier B.V.保留所有权利。 [参考：35]

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