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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Ferricytochrome c encapsulated in silica hydrogels: correlation between active site dynamics and solvent structure
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Ferricytochrome c encapsulated in silica hydrogels: correlation between active site dynamics and solvent structure

机译：包裹在硅胶水凝胶中的亚铁色素c：活性位点动力学与溶剂结构之间的关系

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Ferricytochrome c encapsulated in silica hydrogels has been prepared by the sol-gel technique following, with some modifications, the procedure originally developed by Zink et al. A suitable preparation of hydrogels enables to have both 'wet' and 'dry' samples. Wet samples have a high water content: as the temperature is lowered below similar to260 K water freezes and the samples crack. On the contrary, dry samples have a low water content (hydration h similar to 0.35): in these conditions water does not freeze even at cryogenic temperatures and the samples remain transparent and non-cracking. The dynamics of ferricytochrome c and its dependence on the surrounding medium have been studied by optical absorption spectroscopy in the temperature range 10-300 K. At each temperature, spectra were collected both in the Sorel region and in the near infrared at similar to 1.45 mum (the water overtone band); this enables to probe the local dynamics of the protein active site as well as the 'structure' of water molecules present in the sample. The data show that sol-gel encapsulation 'per se' does not alter the protein active site dynamics, but rather introduces an increased local heterogeneity. At difference. we find a correlation between active site dynamics and water structure: in the wet hydrogel, freezing of water quenches the ensemble of soft modes linearly coupled to the Soret transition; while, in the dry hydrogel, water does not freeze, and an active site dynamic behavior-similar to the non-freezing water/glycerol solution-is observed. (C) 2002 Elsevier Science B.V All rights reserved. [References: 31]
机译：通过溶胶-凝胶技术，按照Zink等人最初开发的方法进行了一些修改，通过溶胶-凝胶技术制备了包裹在二氧化硅水凝胶中的亚铁色素c。合适的水凝胶制剂可以同时具有“湿”和“干”样品。湿样品的水分含量很高：随着温​​度降低到低于260 K，水会冻结并破裂。相反，干燥的样品的水含量低（水合h类似于0.35）：在这些条件下，即使在低温下，水也不会冻结，并且样品保持透明且不开裂。在10-300 K的温度范围内，通过光吸收光谱法研究了亚铁色素c的动力学及其对周围介质的依赖性。在每个温度下，在Sorel区和近红外区均以约1.45 mum的频率收集光谱。 （水泛音带）;这可以探测蛋白质活性位点的局部动力学以及样品中存在的水分子的“结构”。数据表明溶胶-凝胶包封“本身”不会改变蛋白质活性位点的动力学，而是会增加局部异质性。有所不同。我们发现活性位点动力学与水结构之间存在相关性：在湿水凝胶中，水的冻结使线性耦合到Soret跃迁的软模的集合猝灭;而在干燥的水凝胶中，水不会冻结，并且观察到类似于非冻结水/甘油溶液的活性位点动态行为。 （C）2002 Elsevier Science B.V保留所有权利。 [参考：31]

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