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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Complexation of polymethine dyes with human serum albumin: a spectroscopic study.
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Complexation of polymethine dyes with human serum albumin: a spectroscopic study.

机译：聚次甲基染料与人血清白蛋白的络合：光谱研究。

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摘要

Non-covalent interactions between polymethine dyes of various types (cationic and anionic thiacarbocyanines as well as anionic oxonols and tetracyanopolymethines) and human serum albumin (HSA) were studied by means of absorption, fluorescence and circular dichroism (CD) spectroscopies. Complexation with the protein leads to a red shift of the dye absorption spectra and, in most cases, to a growth of the fluorescence quantum yield (Phif; for oxonols this growth is very small). The binding constants (K) obtained from changing the absorption spectra and Phif vary from 10(4) to (5-6) x 10(7) M(-1). K for the anionic dyes is much higher than for the cationic dyes (the highest K was found for oxonols). Interaction of meso-substituted anionic thiacarbocyanines with HSA results in cis-->trans isomerization and, as a consequence, an appearance and a steep rise of dye fluorescence. Binding to HSA gives rise to dye CD signals and in many cases is accompanied by aggregation of the dyes. These aggregates often exhibit biphasic CD spectra. The aggregates formed by the dyes alone are decomposed in the presence of HSA.
机译：通过吸收，荧光和圆二色性（CD）光谱法研究了各种类型的聚次甲基染料（阳离子和阴离子硫杂甲腈，阴离子氧代酚和四氰基聚次甲基）与人血清白蛋白（HSA）之间的非共价相互作用。与蛋白质的络合会导致染料吸收光谱发生红移，并且在大多数情况下会导致荧光量子产率的增长（Phif;对于恶酚，这种增长非常小）。通过改变吸收光谱和Phif获得的结合常数（K）在10（4）至（5-6）×10（7）M（-1）之间变化。阴离子染料的K远远高于阳离子染料的K（氧杂酚的K最高）。介孔取代的阴离子硫杂碳菁与HSA的相互作用导致顺式->反式异构化，因此，染料荧光的出现和急剧增加。与HSA的结合产生染料CD信号，并且在许多情况下伴随着染料的聚集。这些聚集体通常表现出双相CD光谱。仅由染料形成的聚集体在HSA的存在下分解。

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