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首页> 外文期刊>Journal of Molluscan Studies >Method for repeated extrapallial fluid extraction from bivalve molluscs
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Method for repeated extrapallial fluid extraction from bivalve molluscs

机译：从双壳贝类中反复提取颅外液的方法

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摘要

The molluscan extrapallial fluid (EPF) is enclosed between the inner surface of the shell and the mantle. It is used in studies related to a wide range of disciplines, such as biochemistry, geochemistry, environmental chemistry and ecotoxicology. Studying EPF requires its careful extraction from the animal in order to avoid contamination from other fluids, tissues or shell fragments. Extraction of EPF from opened valves using a syringe needle or directly through a hole drilled in the shell valve are common practices. However, chances for EPF contamination are high for these and other known methods. For example, Figure 1 E shows microparticles of shell material found in EPF which was extracted immediately after a hole was drilled through a shell.
机译：软体动物腹腔积液（EPF）被封闭在壳体的内表面和套之间。它用于与许多学科相关的研究，例如生物化学，地球化学，环境化学和生态毒理学。研究EPF需要从动物中小心提取EPF，以避免被其他液体，组织或贝壳碎片污染。常见的做法是使用注射器针头或直接通过壳阀上钻的孔从打开的阀中提取EPF。但是，对于这些和其他已知方法，EPF污染的机会很高。例如，图1E示出了在EPF中发现的壳材料的微粒，其在通过壳钻出孔之后立即被提取。

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