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首页> 外文期刊>Journal of oral biosciences >Porphyromonas gingivalis FimA fimbriae: Roles of the fim gene cluster in the fimbrial assembly and antigenic heterogeneity among fimA genotypes
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Porphyromonas gingivalis FimA fimbriae: Roles of the fim gene cluster in the fimbrial assembly and antigenic heterogeneity among fimA genotypes

机译：牙龈卟啉单胞菌FimA菌毛：fim基因簇在fimA基因型中的纤维装配和抗原异质性中的作用

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摘要

In order for bacteria to exert their pathogenicity, they must adhere to and colonize the host tissues. Porphyromonas gingivalis, a periodontal pathogen, primarily exploits FimA fimbriae for adhesion and colonization. FimA fimbriae are polymers composed of FimA protein encoded by the fimA gene. The fimA gene exists as a single copy within the fim gene cluster. This fim gene cluster contains 7 genes: fimX, pgmA, and fimABCDE. In this article, we address the roles of these genes in fimbrial formation. P. gingivalis strains 381 and ATCC 33277 express long fimbriae several micrometers in length, but we found that the FimB protein is absent in these strains because of a nonsense mutation in the fimB gene. Moreover, we found that FimB restoration resulted in the production of short FimA fimbriae approximately 150 nm in length. These findings indicate that FimB regulates fimbrial length. We also demonstrate that genes in the fim gene cluster, except fimA, are not required for FimA fimbrial assembly. It has been reported that there are several fimA genotypes, and these are associated with differential virulence. As such, we describe a serological analysis of FimA fimbriae across genotypes. We obtained antisera elicited by FimA fimbriae from genotypes I to V. The antisera showed low cross-reactivity between genotypes, indicating that the FimA fimbriae of each genotype were antigenically heterogenic. In addition, the antisera preferentially recognized the polymeric conformation of the fimbriae, which may be responsible for the heterogeneity. Notably, however, the amino acid sequences are partially common among each genotype.
机译：为了使细菌发挥其致病性，它们必须粘附并定殖在宿主组织上。牙龈卟啉单胞菌是一种牙周病原体，主要利用FimA菌毛进行粘附和定居。 FimA菌毛是由fimA基因编码的FimA蛋白组成的聚合物。 fimA基因以单拷贝形式存在于fim基因簇中。这个fim基因簇包含7个基因：fimX，pgmA和fimABCDE。在本文中，我们探讨了这些基因在纤维形成中的作用。牙龈卟啉单胞菌菌株381和ATCC 33277表达的长菌毛长度为几微米，但是我们发现由于fimB基因的无义突变，这些菌株中不存在FimB蛋白。此外，我们发现FimB还原导致长度约150 nm的短FimA菌毛的产生。这些发现表明，FimB调节纤维长度。我们还证明，fimA基因簇中的fim基因簇中的基因（fimA除外）不是必需的。据报道，有几种fimA基因型，这些基因型与致病力有关。因此，我们描述了跨基因型的FimA菌毛的血清学分析。我们从基因型I到V获得了FimA菌毛引起的抗血清。该抗血清在基因型之间显示出较低的交叉反应性，表明每种基因型的FimA菌毛都是抗原异源的。此外，抗血清优先识别菌毛的聚合构象，这可能是异质性的原因。然而，值得注意的是，氨基酸序列在每种基因型之间是部分共有的。

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