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首页> 外文期刊>Journal of neurology >Serum free hemoglobin as a novel potential biomarker for acute ischemic stroke.
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Serum free hemoglobin as a novel potential biomarker for acute ischemic stroke.

机译：无血清血红蛋白作为急性缺血性中风的新型潜在生物标志物。

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OBJECTIVE: Currently, no practical biomarker is available for the diagnosis of acute ischemic stroke. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has being applied extensively to analyze large biomolecules such as proteins. The technique is likely to be of remarkable value as indicators of systemic processes such as atherosclerosis and stroke. The aim of this study is to identify potential protein biomarkers for ischemic stroke diagnosis utilizing MALDI-TOF MS. METHODS: Serum samples obtained from acute ischemic stroke patients (n = 47) and controls (n = 34) were analyzed by MALDI-TOF MS. Stroke severity was assessed by the National Institutes of Health Stroke Scale (NIHSS), subtype by the Trial of Org 10172 in Acute Stroke Treatment (TOAST), functional outcome by the modified Rankin Scale (mRS) and infarct volume by the diffusion-weighted images. Risk factors and routine laboratory data of the stroke patients were registered prospectively. RESULTS: The peaks of hemoglobin (Hb) alpha-chain and beta-chain were differentially expressed between stroke patients and controls (p < 0.0001). Hb ions were detected in the samples collected from 33 (70.2%) stroke patients and 5 (14.7%) controls. The sensitivity is 70.2% and the specificity is 85.3%. Among stroke patients, there is no significant correlation (p > 0.05) between Hb peaks and the NIHSS, TOAST, mRS, stroke risk factors, infarct volume, infarct location and laboratory data. CONCLUSIONS: Serum free Hb may serve as a novel potential biomarker for the diagnosis of acute ischemic stroke. The clinical value of this potential biomarker may be clarified by further studies quantifying serum free Hb levels.
机译：目的：目前尚无可用于诊断急性缺血性中风的实用生物标志物。基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）已广泛应用于分析大型生物分子，例如蛋白质。该技术作为动脉粥样硬化和中风等全身过程的指标可能具有非凡的价值。这项研究的目的是利用MALDI-TOF MS识别用于缺血性中风诊断的潜在蛋白质生物标志物。方法：通过MALDI-TOF MS分析急性缺血性中风患者（n = 47）和对照组（n = 34）的血清样本。中风严重程度由美国国立卫生研究院中风量表（NIHSS）评估，亚型10172急性中风治疗试验（TOAST）评估亚型，功能结局由改良的兰金量表（mRS）和弥散加权图像评估梗死体积。前瞻性地登记了中风患者的危险因素和常规实验室数据。结果：中风患者和对照组之间的血红蛋白（Hb）α-链和β-链的峰差异表达（p <0.0001）。从33名（70.2％）中风患者和5名（14.7％）对照收集的样品中检测到Hb离子。灵敏度为70.2％，特异性为85.3％。在中风患者中，Hb峰值与NIHSS，TOAST，mRS，中风危险因素，梗塞体积，梗塞位置和实验室数据之间无显着相关性（p> 0.05）。结论：血清游离Hb可作为诊断急性缺血性中风的新型潜在生物标志物。这种潜在的生物标志物的临床价值可能会通过进一步研究量化血清游离Hb水平来阐明。

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