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首页> 外文期刊>Journal of neurology >Increased prevalence of and gene transcription by Chlamydia pneumoniae in cerebrospinal fluid of patients with relapsing-remitting multiple sclerosis.
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Increased prevalence of and gene transcription by Chlamydia pneumoniae in cerebrospinal fluid of patients with relapsing-remitting multiple sclerosis.

机译：复发-缓解型多发性硬化症患者脑脊液中肺炎衣原体的患病率和基因转录增加。

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Microbial agents may play a role in the pathogenesis of multiple sclerosis (MS). C. pneumoniae has been recently associated with MS; however, study results are at variance. We tested the hypothesis that Chlamydia pneumoniae-specific DNA and RNA are more often detected in cerebrospinal fluid (CSF) of patients with multiple sclerosis than patients with other neurological diseases (OND). We investigated CSF samples from 84 patients with definite MS and 89 OND patients (n = 62 with normal CSF; n = 27 with pathological CSF) using a nested polymerase chain reaction (PCR) to detect ompA gene sequences of C. pneumoniae. In subjects with positive PCR, we probed for chlamydial heat shock protein 60-mRNA and 16S-rRNA by reverse transcriptase (rt)-PCR. C. pneumoniae-specific DNA was more often detected in MS patients (50 %) than in all OND patients combined (28.1%, p = 0.003) and in OND patients with normal CSF (24.2%, p = 0.003) but not than in OND patients with pathological CSF (37%, p = 0.24). In relapsing-remitting MS (n = 55), the prevalence of C. pneumoniae DNA was higher (66.7 %) than in both OND subgroups (p
机译：微生物制剂可能在多发性硬化症（MS）的发病机理中起作用。肺炎衣原体最近与MS有关。但是，研究结果不一致。我们检验了以下假设，即多发性硬化症患者的脑脊液（CSF）比其他神经系统疾病（OND）的患者更常检测到肺炎衣原体特异的DNA和RNA。我们使用巢式聚合酶链反应（PCR）研究了84例MS确诊患者和89例OND患者（n = 62例正常CSF; n = 27例病理CSF）的CSF样本，以检测肺炎衣原体的ompA基因序列。在具有阳性PCR的受试者中，我们通过逆转录酶（rt）-PCR检测了衣原体热休克蛋白60-mRNA和16S-rRNA。在MS患者（50％）中检出肺炎衣原体特异性DNA的频率高于所有OND患者的总和（28.1％，p = 0.003）和CSF正常的OND患者（24.2％，p = 0.003）但未检出病理性脑脊液的OND患者（37％，p = 0.24）。在复发型多发性硬化症患者中（n = 55），肺炎衣原体DNA的患病率（66.7％）高于两个OND亚组（p≤0.05）。 MS患者（n = 20）中检出的衣原体热休克蛋白60-mRNA（75％）和16S-rRNA（70％）比OND患者（n = 16; 18.8％; p <0.005）更多。 CSF中的肺炎衣原体DNA由于在OND对照中普遍存在，因此在多发性复发性MS中更常检测到，CSF中的肺炎衣原体DNA对MS不具有特异性。但是，较高的基因转录率表明MS患者肺炎衣原体的代谢更活跃。

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