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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >The different roles of metal ions and water molecules in the recognition and catalyzed hydrolysis of ATP by phenanthroline-containing polyamines
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The different roles of metal ions and water molecules in the recognition and catalyzed hydrolysis of ATP by phenanthroline-containing polyamines

机译：金属离子和水分子在含菲咯啉的多胺识别和催化ATP水解中的不同作用

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The phenanthroline bridging polyaza ligands L1, L2 and L3 can selectively and strongly bind nucleotides at physiological pH, and hence accelerate the hydrolysis rate of the bound ATP. It is interesting that a phosphoramidate intermediate at 2.88 ppm (should be added 5.63 ppm when compared with other models) was found in the hydrolysis process of L/ATP. By introduction of metal ions (critical Zn2+ or hard Mg2+, Ca2+) to the L/ATP system, recognition of the anionic substrates by the protonated ligands was greatly promoted. However, due to the different affinities of metal ions to the receptor and the substrate, ATP hydrolysis in Zn2+/L/ATP system and Mg2+(Ca2+)/L/ATP system occurs through different mechanisms. By comparison with the M/ATP (M = Zn2+, Mg2+, Ca2+) system, the rates of ATP-hydrolysis in the Mg2+ Ca2+/L/ATP system and the Zn2+/L/ATP system were enhanced and retarded, respectively. Moreover, the reasons contributing to large rate range of the L/ATP systems and M2+/L/ATP systems were given. The results show that metal ions vertically regulate the recognition and hydrolysis of ATP. On the other hand, water molecule participates in the hydrolysis reactions at different steps with different functions in the L/ATP systems and M(Zn2+, Mg2+, Ca2+)L/ATP systems. (C) 2003 Elsevier B.V. All rights reserved. [References: 32]
机译：菲咯啉桥联的聚氮杂配体L1，L2和L3可以在生理pH下选择性和牢固地结合核苷酸，从而加快结合ATP的水解速度。有趣的是，在L / ATP的水解过程中发现了2.88 ppm的氨基磷酸酯中间体（与其他模型相比应添加5.63 ppm）。通过将金属离子（临界Zn2 +或硬Mg2 +，Ca2 +）引入L / ATP系统，可以大大促进质子化配体对阴离子底物的识别。但是，由于金属离子对受体和底物的亲和力不同，Zn2 + / L / ATP系统和Mg2 +（Ca2 +）/ L / ATP系统中的ATP水解机制不同。通过与M / ATP（M = Zn2 +，Mg2 +，Ca2 +）体系进行比较，Mg2 + Ca2 + / L / ATP体系和Zn2 + / L / ATP体系中ATP的水解速率分别得到提高和降低。此外，给出了导致L / ATP系统和M2 + / L / ATP系统速率范围较大的原因。结果表明，金属离子垂直调节ATP的识别和水解。另一方面，在L / ATP系统和M（Zn2 +，Mg2 +，Ca2 +）L / ATP系统中，水分子以不同的功能参与不同的水解反应。 （C）2003 Elsevier B.V.保留所有权利。 [参考：32]

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