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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >On the stiffness of the natural actin filament decorated with alexa fluor tropomyosin
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On the stiffness of the natural actin filament decorated with alexa fluor tropomyosin

机译:关于用Alexa荧光原肌球蛋白修饰的天然肌动蛋白丝的刚度

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摘要

Natural, phalloidin-free, actin filaments were decorated with tropomyosin made fluorescent by reaction with alexa fluor (R) 488 C-5 maleimide. The elastic modulus by stretching of these filaments was then determined and found to span between 38.2 MPa and 61.48 MPa. We tried also to determine the yield strength of the same filaments in the laser light trap operated at 920 mW, the maximum power of the apparatus. Only two out of the 10 filaments tested were broken under these conditions, yield strength being 50.5 and 55 pN, respectively. (C) 2003 Elsevier Science B.V. All rights reserved. [References: 26]
机译:天然的,不含鬼笔环肽的肌动蛋白丝用通过与alexa fluor(R)488 C-5马来酰亚胺反应而发荧光的原肌球蛋白修饰。然后确定了通过拉伸这些细丝的弹性模量,发现其跨度在38.2MPa至61.48MPa之间。我们还试图确定以920 mW(设备的最大功率)工作的激光陷阱中相同灯丝的屈服强度。在这些条件下,测试的10条细丝中只有2条断裂,屈服强度分别为50.5和55 pN。 (C)2003 Elsevier Science B.V.保留所有权利。 [参考:26]

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