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首页> 外文期刊>Journal of orthopaedic research >Growth factor and protease expression during different phases of healing after rabbit deep flexor tendon repair.
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Growth factor and protease expression during different phases of healing after rabbit deep flexor tendon repair.

机译：兔深屈肌腱修复后不同阶段愈合中的生长因子和蛋白酶表达。

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The purpose of the study was to contribute to the mapping of molecular events during flexor tendon healing, in particular the growth factors insulin-like growth factor-1 (IGF-1), vascular endothelial growth factor (VEGF) and nerve growth factor (NGF), matrix metalloproteinases (MMP-3 and MMP-13) and their inhibitors (tissue inhibitors of metalloproteinases, TIMP-1 and TIMP-3, and the protease cathepsin K. In a rabbit model of flexor tendon injury, the mRNA expression for the growth factors, MMPs and TIMPs were measured in tendon and tendon sheath tissue at several time points (3, 6, 21, and 42 days) representing different phases of the healing process. We found that MMP-13 remained increased during the study period, whereas MMP-3 returned to normal levels within the first week after injury. TIMP-3 was down-regulated in the tendon sheaths. Cathepsin K was up-regulated in tendons and sheaths after injury. NGF was present in both tendons and sheaths, but unaltered. IGF-1 exhibited a late increase in the tendons, while VEGF was down-regulated at the later time points. In conclusion, we have demonstrated the presence of NGF in flexor tendons. MMP-13 expression appears to play a more protracted role in flexor tendon healing than MMP-3. The relatively low levels of endogenous IGF-1 and VEGF mRNA following injury support their potential beneficial role as exogenous modulators to optimize tendon healing and strength without increasing adhesion formation.
机译：这项研究的目的是有助于绘制屈肌腱愈合期间的分子事件，特别是胰岛素样生长因子-1（IGF-1），血管内皮生长因子（VEGF）和神经生长因子（NGF）等生长因子），基质金属蛋白酶（MMP-3和MMP-13）及其抑制剂（金属蛋白酶的组织抑制剂TIMP-1和TIMP-3和蛋白酶组织蛋白酶K）。在兔屈肌腱损伤模型中，mRNA的表达在代表愈合过程不同阶段的多个时间点（3、6、21和42天）测量了肌腱和腱鞘组织中的生长因子，MMP和TIMP，我们发现在研究期间MMP-13仍保持增加，而MMP-3在损伤后的第一周内恢复到正常水平; TIMP-3在肌腱鞘中被下调;组织蛋白酶K在肌腱和鞘中在受伤后被上调; NGF在肌腱和鞘中均存在，但IGF-1表现出晚期肌腱增加，而VEGF在随后的时间点被下调。总之，我们证明了屈肌腱中存在NGF。 MMP-13的表达似乎在屈肌腱愈合中比MMP-3发挥更持久的作用。损伤后内源性IGF-1和VEGF mRNA相对较低的水平支持了它们作为外源性调节剂的潜在有益作用，可在不增加粘连形成的情况下优化肌腱愈合和强度。

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