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首页> 外文期刊>Journal of orthopaedic research >Needle puncture injury causes acute and long-term mechanical deficiency in a mouse model of intervertebral disc degeneration
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Needle puncture injury causes acute and long-term mechanical deficiency in a mouse model of intervertebral disc degeneration

机译：针刺损伤在椎间盘退变的小鼠模型中引起急性和长期的机械缺陷

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Low back pain is a significant socioeconomic burden and intervertebral disc degeneration has been implicated as a cause. A reliable animal model of disc degeneration is necessary to evaluate therapeutics, and functional metrics are essential to quantify their benefit. To this end, needle puncture injuries were created in the caudal intervertebral discs of mice to induce disc degeneration. Compression, torsion, and creep mechanics were assessed both immediately and after eight weeks to distinguish between the effects of injury and the subsequent reparative or degenerative response. Two needle sizes (29 and 26 gauge) were used to determine injury size-dependence. Compressive stiffness (62%), torsional stiffness (60%), and early damping stiffness (84%) decreased immediately after injury with the large needle (26G). These mechanical properties did not change over time despite structural and compositional changes. At 8 weeks following large needle injury, disc height decreased (37%), nucleus pulposus (NP) glycosaminoglycan content decreased (41%), and NP collagen content increased (45%). The small needle size had no significant effect on mechanics and did not initiate degenerative changes in structure and composition. Thus, the injection of therapeutics into the NP with a minimal needle size may limit damage due to the needle insertion. These findings, along with the wide commercial availability of mouse-specific biological probes, indicate that the mouse caudal disc model can be a powerful tool for investigating disc degeneration and therapy.
机译：腰背痛是重大的社会经济负担，并且椎间盘退变已被认为是原因。椎间盘退变的可靠动物模型对于评估治疗方法必不可少，而功能指标对于量化其获益至关重要。为此，在小鼠的尾椎间盘中产生了针刺损伤，以引起盘变性。立即评估压迫，扭转和蠕变力学，并在八周后进行评估，以区分伤害的影响和随后的修复或退化反应。使用两种针头尺寸（29和26号针规）来确定损伤尺寸的依赖性。大针头（26G）受伤后，抗压刚度（62％），扭转刚度（60％）和早期阻尼刚度（84％）下降。尽管结构和成分有所变化，但这些机械性能并未随时间变化。大针刺伤后8周，椎间盘高度降低（37％），髓核（NP）糖胺聚糖含量降低（41％），NP胶原蛋白含量增加（45％）。小号的针头对机械性能没有显着影响，并且不会引起结构和成分的退化性变化。因此，以最小的针头尺寸将治疗剂注入NP可能会限制由于针头插入而造成的损坏。这些发现以及小鼠特异性生物探针的广泛商业应用表明，小鼠尾椎间盘模型可以成为研究椎间盘退变和治疗的有力工具。

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