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首页> 外文期刊>Journal of orthopaedic research >Atomic force microscopy reveals regional variations in the micromechanical properties of the pericellular and extracellular matrices of the meniscus
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Atomic force microscopy reveals regional variations in the micromechanical properties of the pericellular and extracellular matrices of the meniscus

机译：原子力显微镜揭示了半月板细胞周围和细胞外基质的微机械特性的区域变化

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摘要

Regional variations in the composition and architecture of the extracellular matrix (ECM) and pericellular matrix (PCM) of the knee meniscus play important roles in determining the local mechanical environment of meniscus cells. In this study, atomic force microscopy was used to spatially map the mechanical properties of matched ECM and perlecan-labeled PCM sites within the outer, middle, and inner porcine medial meniscus, and to evaluate the properties of the proximal surface of each region. The elastic modulus of the PCM was significantly higher in the outer region (151.4 ± 38.2 kPa) than the inner region (27.5 ± 8.8 kPa), and ECM moduli were consistently higher than region-matched PCM sites in both the outer (320.8 ± 92.5 kPa) and inner (66.1 ± 31.4 kPa) regions. These differences were associated with a higher proportion of aligned collagen fibers and lower glycosaminoglycan content in the outer region. Regional variations in the elastic moduli and some viscoelastic properties were observed on the proximal surface of the meniscus, with the inner region exhibiting the highest moduli overall. These results indicate that matrix architecture and composition play an important role in the regional micromechanical properties of the meniscus, suggesting that the local stress-strain environment of meniscal cells may vary significantly among the different regions.
机译：膝盖半月板的细胞外基质（ECM）和细胞周围基质（PCM）的成分和结构的区域变化在确定半月板细胞的局部机械环境中起重要作用。在这项研究中，原子力显微镜被用来在空间上绘制猪半月板内侧，内侧和内侧半月板内匹配的ECM和经珍珠岩标记的PCM部位的机械性能，并评估每个区域近端表面的性能。 PCM的弹性模量在外部区域（151.4±38.2 kPa）显着高于内部区域（27.5±8.8 kPa），并且ECM模量在两个外部区域（320.8±92.5）始终高于区域匹配的PCM位置。 kPa）和内部（66.1±31.4 kPa）区域。这些差异与对齐的胶原纤维比例较高和外部区域糖胺聚糖含量较低有关。在弯月面的近端表面上观察到了弹性模量的区域变化和一些粘弹性质，内部区域总体上表现出最高的模量。这些结果表明，基质结构和组成在半月板的区域微机械性能中起重要作用，表明半月板细胞的局部应力-应变环境在不同区域之间可能有显着变化。

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