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首页> 外文期刊>Journal of orthopaedic research >Crosslinking density influences the morphology of chondrocytes photoencapsulated in PEG hydrogels during the application of compressive strain.
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Crosslinking density influences the morphology of chondrocytes photoencapsulated in PEG hydrogels during the application of compressive strain.

机译：交联密度会影响在施加压缩应变期间光包裹在PEG水凝胶中的软骨细胞的形态。

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Chondrocyte deformation, which occurs during mechanical loading, is thought to play an important role in the mechanotransduction pathway. In designing a scaffold that can be gelled in situ for cartilage tissue engineering, an important consideration is the influence of mechanical loading. This study tested the hypothesis that changes in the crosslinking density of a hydrogel scaffold influence the morphology of encapsulated chondrocytes in response to an applied load. Chondrocytes were entrapped in photocrosslinkable hydrogel scaffolds based on poly(ethylene glycol) (PEG) with two crosslinking densities, 0.119 and 0.376 mol/l, with the higher density having a 11-fold higher compressive modulus. The cell-embedded hydrogels were subjected to static compressive strains between 0% and 20% after 1 and 6 days of culture. Using confocal laser scanning microscopy, chondrocytes in the highly crosslinked gel at day 1 deformed more than gels in the more loosely crosslinked gel. By day 6, this finding was reversed. When single cells within a region were followed, heterogeneities in cell deformation were observed on both a macroscopic and microscopic scale. These heterogeneities were greater in the highly crosslinked gel. These findings demonstrate that different levels of cell deformation and heterogeneity may be obtained by varying the crosslinking density in PEG hydrogels.
机译：在机械负荷过程中发生的软骨细胞变形被认为在机械转导途径中起重要作用。在设计可以原位凝胶化的软骨组织工程支架时，重要的考虑因素是机械载荷的影响。这项研究检验了以下假设：水凝胶支架的交联密度的变化会影响所施加的负荷，从而影响封装的软骨细胞的形态。软骨细胞被困在具有两种交联密度0.119和0.376 mol / l的基于聚（乙二醇）（PEG）的光可交联水凝胶支架中，密度越高，压缩模量高11倍。培养1至6天后，将嵌入细胞的水凝胶经受0％至20％之间的静态压缩应变。使用共聚焦激光扫描显微镜，第1天高度交联的凝胶中的软骨细胞比松散交联的凝胶中的凝胶变形更大。到第6天，这一发现被推翻了。当跟随一个区域内的单个细胞时，在宏观和微观尺度上都观察到了细胞变形的异质性。在高度交联的凝胶中，这些异质性更大。这些发现表明，通过改变PEG水凝胶中的交联密度，可以获得不同水平的细胞变形和异质性。

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