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首页> 外文期刊>Journal of orthopaedic research >Pulsed electromagnetic fields stimulation affects osteoclast formation by modulation of osteoprotegerin, RANK ligand and macrophage colony-stimulating factor.
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Pulsed electromagnetic fields stimulation affects osteoclast formation by modulation of osteoprotegerin, RANK ligand and macrophage colony-stimulating factor.

机译：脉冲电磁场​​刺激通过调节骨保护素，RANK配体和巨噬细胞集落刺激因子来影响破骨细胞的形成。

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Electromagnetic stimulation has been documented to treat recalcitrant problems of musculoskeletal system. Yet, the underlying mechanisms are not completely understood. In this study, we investigated effect of pulsed electromagnetic fields (PEMF) with parameters modified from clinical bone growth stimulator on osteoclast formation, bone resorption, and cytokines associated with osteoclastogenesis. Marrow cells were harvested from both femora and tibiae of 6 week-old mice and cultured in 8-well chamber slides or 16-well calcium phosphate apatite-coated multitest slides. After 1-day incubation, marrow cells were exposed to PEMF at different electric field intensities for 2h/day and continued for 9 days. Osteoprotegerin (OPG), receptor activator of NFkappaB-ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) concentrations of each group were determined after PEMF stimulation. Osteoclast identity was confirmed by both tartrate resistant acid phosphatase (TRAP) stain and bone resorption assay. A statistically significant increase and decrease of osteoclastogenesis and bone resorption areas were found when exposed to PEMF with different intensities. Besides, consistent correlations among OPG, RANKL, M-CSF, osteoclast numbers, and bone resorption after exposure to different intensities of PEMF were observed. These data demonstrated that PEMF with different intensities could regulate osteoclastogenesis, bone resorption, OPG, RANKL, and M-CSF concentrations in marrow culture system.
机译：电磁刺激已被证明可以治疗肌肉骨骼系统的顽疾。但是，尚未完全理解其基本机制。在这项研究中，我们调查了脉冲电磁场​​（PEMF），其参数从临床骨生长刺激剂修改后对破骨细胞形成，骨吸收和与破骨细胞形成相关的细胞因子的影响。从6周龄小鼠的股骨和胫骨中收获骨髓细胞，并在8孔室玻片或16孔磷酸钙磷灰石包被的多试验玻片中培养。温育1天后，将骨髓细胞在不同电场强度下暴露于PEMF 2小时/天，并持续9天。在PEMF刺激后，测定每组的骨保护素（OPG），NFκB-配体的受体激活剂（RANKL）和巨噬细胞集落刺激因子（M-CSF）的浓度。酒石酸抗性酸性磷酸酶（TRAP）染色和骨吸收试验均证实了破骨细胞的身份。当暴露于不同强度的PEMF时，发现破骨细胞生成和骨吸收区域的统计学显着增加和减少。此外，在暴露于不同强度的PEMF后，OPG，RANKL，M-CSF，破骨细胞数量和骨吸收之间存在一致的相关性。这些数据表明，不同强度的PEMF可以调节骨髓培养系统中的破骨细胞生成，骨吸收，OPG，RANKL和M-CSF浓度。

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