...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of orthopaedic research >Nondestructive evaluation of osteogenic differentiation in tissue-engineered constructs.
【24h】

Nondestructive evaluation of osteogenic differentiation in tissue-engineered constructs.

机译：组织工程构造中成骨分化的非破坏性评估。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Conventional measurements of osteogenesis in tissue-engineered constructs are destructive to living cells and incapable to provide three-dimensional information. In the present study, noninvasive magnetic resonance (MR) microscopy was used to evaluate osteogenic differentiation in vitro in human mesenchymal stem cell-based tissue-engineered constructs. The constructs were prepared by seeding the cells (10(6)cells/ml) on 4 x 4 x 4 mm gelatin sponge cubes and subsequently exposing them to osteogenic differentiation or basic medium. During the 4-week experiment, alkaline phosphatase (ALP) activity and calcium content of differentiated constructs were significantly increased compared to the basic medium controls. The T1, T2, and apparent diffusion coefficient (ADC) of differentiated constructs were significantly lower than those of the control group at each time point (p < 0.05). The MR parameters of constructs were significantly correlated to their ALP activities (r to T1, T2, and ADC = -0.57, -0.78, and -0.81, respectively) and calcium content (r to T1, T2, and ADC = 0.48, 0.90, and 0.92, respectively) measured by biochemical techniques. MR microscopy can be a promising tool for noninvasive assessment of osteogenic differentiation and to provide three-dimensional information about tissue-engineered constructs.
机译：组织工程构造中成骨作用的常规测量结果对活细胞具有破坏性，无法提供三维信息。在本研究中，无创磁共振（MR）显微镜用于评估人间充质干细胞为基础的组织工程构建体中的成骨分化。通过将细胞（10（6）个细胞/ ml）接种在4 x 4 x 4 mm明胶海绵立方体上，然后将其暴露于成骨细胞分化或碱性培养基中来制备构建体。在4周的实验过程中，与基本培养基对照组相比，分化后构建体的碱性磷酸酶（ALP）活性和钙含量显着提高。在每个时间点，分化构建体的T1，T2和表观扩散系数（ADC）均显着低于对照组（p <0.05）。构造物的MR参数与它们的ALP活性（r分别为T1，T2和ADC分别为-0.57，-0.78和-0.81）和钙含量（r分别为T1，T2和ADC = 0.48、0.90）显着相关，和分别为0.92）。 MR显微镜可能是用于成骨分化的非侵入性评估并提供有关组织工程构造的三维信息的有前途的工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号