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首页> 外文期刊>Journal of orthopaedic research >Effects of sintered bovine bone on cell proliferation, collagen synthesis, and osteoblastic expression in MC3T3-E1 osteoblast-like cells.
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Effects of sintered bovine bone on cell proliferation, collagen synthesis, and osteoblastic expression in MC3T3-E1 osteoblast-like cells.

机译：牛骨烧结对MC3T3-E1成骨细胞样细胞增殖，胶原合成和成骨细胞表达的影响。

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摘要

To determine the function of biomaterials on osteoblasts, we studied the effects of two different biomaterials, sintered bovine bone (true bone ceramic) and hydroxyapatite-related material, on DNA and collagen synthesis and osteoblastic expression in osteoblast-like cells in vitro. Osteoblasts cultured with true bone ceramic exhibited greater increases in DNA and collagenous protein synthesis and alkaline phosphatase activity than those cultured with hydroxyapatite. Furthermore, expression of mRNA for type-I collagen and osteocalcin, as assessed by reverse transcription-polymerase chain reaction, was higher in cultures grown on true bone ceramic or hydroxyapatite than in those grown with glass fragments, and levels of gene expression in true bone ceramic and hydroxyapatite cultures were at almost the same level. These findings indicate that osteoblasts cultured with true bone ceramic increase their activity, suggesting that true bone ceramic may be a more favorable substrate than hydroxyapatite for growth and differentiation of osteoblast-like cells.
机译：为了确定生物材料在成骨细胞中的功能，我们研究了两种不同的生物材料，烧结牛骨（真骨陶瓷）和羟基磷灰石相关材料，对体外成骨细胞样细胞中DNA和胶原蛋白合成以及成骨细胞表达的影响。与用羟基磷灰石培养的成骨细胞相比，用真骨陶瓷培养的成骨细胞在DNA和胶原蛋白合成以及碱性磷酸酶活性方面表现出更大的增加。此外，通过逆转录-聚合酶链反应评估，在真骨陶瓷或羟基磷灰石上生长的培养物中，I型胶原蛋白和骨钙素的mRNA表达高于玻璃碎片培养的培养物中，并且在真骨中的基因表达水平更高陶瓷和羟基磷灰石的培养物几乎处于同一水平。这些发现表明，用真骨陶瓷培养的成骨细胞增加了它们的活性，表明对于骨样细胞的生长和分化，与羟基磷灰石相比，真骨陶瓷可能是更有利的基质。

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