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首页> 外文期刊>Journal of orthopaedic research >Responsiveness of bovine chondrocytes to growth factors in medium with different serum concentrations.
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Responsiveness of bovine chondrocytes to growth factors in medium with different serum concentrations.

机译：在不同血清浓度的培养基中，牛软骨细胞对生长因子的反应性。

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Autologous transplantation of chondrocytes is currently under investigation as a potential therapy to stimulate intrinsic repair in articular cartilage defects. The quality of the repair tissue may benefit from the preservation of the characteristic chondrocytic phenotype of the transplanted cells together with the production of a new extracellular matrix composed of collagen type II and larger proteoglycans. A number of growth factors are believed to play an important role in the process of generating new cartilage repair tissue. In this study, the dose-dependent response of bovine chondrocytes to recombinant human insulin-like growth factor-1, recombinant human transforming growth factor-beta2, and recombinant human bone morphogenetic protein-2 was studied in an alginate culture system under different culture conditions. The chondrocytes were cultured in medium with increasing concentrations of fetal calf serum. The cultures were assessed by the total amount of DNA, quantitative and qualitative synthesis of proteoglycan, production of nitric oxide, and histology. Cells cultured in the presence of each growth factor had an equal, nonsignificant stimulation of DNA synthesis compared with those cultured in basal medium alone. Recombinant human insulin-like growth factor-1 and recombinant human transforming growth factor-beta2 stimulated proteoglycan synthesis in a dose-dependent and reversed dose-dependent fashion, respectively. Recombinant human bone morphogenetic protein-2 stimulated proteoglycan synthesis significantly only in the absence of fetal calf serum or in the presence of small amounts of the serum. Overall, proteoglycan synthesis dramatically decreased with the addition of each growth factor as the concentration of fetal calf serum in the medium decreased, and the dose-dependent stimulation pattern, as observed for recombinant human insulin-like growth factor-1 and recombinant human transforming growth factor-beta2, disappeared. Apart from a moderate increase in mRNA for aggrecan and decorin, the growth factors did not greatly affect the type of proteoglycans synthesized. Histological examination confirmed the presence of a dense pericellular matrix deposition, especially when the chondrocytes were cultured in the presence of recombinant human insulin-like growth factor-1 or recombinant human transforming growth factor-beta2. The results indicate that these growth factors can stimulate qualitatively superior matrix production and that the responsiveness of the chondrocytes to the growth factors changes with the culture conditions. Further knowledge about the interaction between chondrocytes, growth factors, and the external environment is important to stimulate chondrocytes to produce adequate repair tissue in cartilage defects in vivo. Insulin-like growth factor-1 especially seems capable of stimulating, in the most consistent and predictable fashion, qualitatively superior proteoglycan synthesis by differentiated chondrocytes. Additional in vivo studies are needed to evaluate the potential of these growth factors as stimulators in cartilage repair.
机译：软骨细胞的自体移植目前正在研究中，作为刺激关节软骨缺损内在修复的潜在疗法。修复组织的质量可能得益于移植细胞特征性软骨细胞表型的保存以及新的由II型胶原蛋白和较大蛋白聚糖组成的细胞外基质的生产。据信许多生长因子在产生新的软骨修复组织的过程中起重要作用。在这项研究中，在不同培养条件下，在藻酸盐培养系统中研究了牛软骨细胞对重组人胰岛素样生长因子-1，重组人转化生长因子β2和重组人骨形态发生蛋白-2的剂量依赖性反应。 。软骨细胞在胎牛血清浓度增加的培养基中培养。通过DNA总量，蛋白聚糖的定量和定性合成，一氧化氮的产生以及组织学来评估培养物。与仅在基础培养基中培养的细胞相比，在每种生长因子存在下培养的细胞对DNA合成的刺激作用均等，无显着性。重组人胰岛素样生长因子-1和重组人转化生长因子β2分别以剂量依赖性和反向剂量依赖性方式刺激蛋白聚糖合成。仅在不存在胎牛血清或存在少量血清的情况下，重组人骨形态发生蛋白2才能显着刺激蛋白聚糖的合成。总体上，随着培养基中胎牛血清浓度的降低，蛋白聚糖的合成随着添加每种生长因子而显着下降，并且重组人胰岛素样生长因子-1和重组人转化生长所观察到的剂量依赖性刺激模式β2因子消失。除了聚集蛋白聚糖和decorin mRNA的适度增加外，生长因子并未对合成蛋白聚糖的类型产生很大影响。组织学检查证实存在致密的细胞周基质沉积，特别是当在重组人胰岛素样生长因子-1或重组人转化生长因子β2存在下培养软骨细胞时。结果表明，这些生长因子可以在质量上刺激基质的产生，并且软骨细胞对生长因子的响应性随培养条件而变化。对软骨细胞，生长因子和外部环境之间相互作用的进一步了解对于刺激软骨细胞在体内软骨缺损中产生足够的修复组织非常重要。胰岛素样生长因子-1似乎能够以最一致和可预测的方式刺激分化软骨细胞在质上优于蛋白聚糖的合成。需要更多的体内研究来评估这些生长因子在软骨修复中作为刺激物的潜力。

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