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首页> 外文期刊>Journal of orthopaedic research >Cartilage tissue engineering by expanded goat articular chondrocytes.
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Cartilage tissue engineering by expanded goat articular chondrocytes.

机译：通过扩大的山羊关节软骨细胞进行软骨组织工程。

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In this study we investigated whether expanded goat chondrocytes have the capacity to generate cartilaginous tissues with biochemical and biomechanical properties improving with time in culture. Goat chondrocytes were expanded in monolayer with or without combinations of FGF-2, TGF-beta1, and PDGFbb, and the postexpansion chondrogenic capacity assessed in pellet cultures. Expanded chondrocytes were also cultured for up to 6 weeks in HYAFF-M nonwoven meshes or Polyactive foams, and the resulting cartilaginous tissues were assessed histologically, biochemically, and biomechanically. Supplementation of the expansion medium with FGF-2 increased the proliferation rate of goat chondrocytes and enhanced their postexpansion chondrogenic capacity. FGF-2-expanded chondrocytes seeded in HYAFF-M or Polyactive scaffolds formed cartilaginous tissues with wet weight, glycosaminoglycan, and collagen content, increasing from 2 days to 6 weeks culture (up to respectively 2-, 8-, and 41-fold). Equilibrium and dynamic stiffness measured in HYAFF M-based constructs also increased with time, up to, respectively, 1.3- and 16-fold. This study demonstrates the feasibility to engineer goat cartilaginous tissues at different stages of development by varying culture time, and thus opens the possibility to test the effect of maturation stage of engineered cartilage on the outcome of cartilage repair in orthotopic goat models.
机译：在这项研究中，我们调查了扩张的山羊软骨细胞是否具有生成软骨组织的能力，其生化和生物力学特性会随着培养时间的延长而改善。山羊软骨细胞在有或没有FGF-2，TGF-beta1和PDGFbb的组合下单层扩增，并且在沉淀培养中评估了扩增后的软骨形成能力。还可以在HYAFF-M非织造网或Polyactive泡沫中培养膨胀的软骨细胞长达6周，并通过组织学，生化和生物力学评估所得的软骨组织。补充FGF-2的膨胀培养基可提高山羊软骨细胞的增殖速率，并增强其膨胀后的软骨形成能力。接种在HYAFF-M或多活性支架中的FGF-2膨胀软骨细胞形成了具有湿重，糖胺聚糖和胶原蛋白含量的软骨组织，培养时间从2天增加到6周（分别达到2倍，8倍和41倍） 。在基于HYAFF M的构造中测得的平衡度和动态刚度也随时间增加，分别达到1.3倍和16倍。这项研究证明了通过改变培养时间在不同发育阶段对山羊软骨组织进行工程改造的可行性，从而为在原位山羊模型中测试工程化软骨成熟阶段对软骨修复结果的影响开辟了可能性。

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