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首页> 外文期刊>Journal of orthopaedic research >In vitro model of full-thickness cartilage defect healing.
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In vitro model of full-thickness cartilage defect healing.

机译：全层软骨缺损愈合的体外模型。

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摘要

Integration of the host-graft interface is implicated as one of the significant reasons for lack of complete healing in osteochondral grafting procedures for the treatment of cartilage lesions. We developed an in vitro model of cartilage healing in an osteochondral setting to study the effect of developmental age and collagenase treatment. Circular full-thickness vertical surgical incisions were made in the cartilaginous portion of cylindrical bovine osteochondral specimens. Two age groups were selected: Young (1-2 months old) and Older (6-8 months old). Cartilage integration across the surgical incisions was assessed by histologic analysis and by mechanical push-out testing at 2 and 4 weeks in culture. Histologic integration as well as peak push-out shear stress was significantly higher in older calf cartilage than in the young calf. Collagenase pretreatment in the older calf samples increased push-out strength at 4 weeks. Histologic integration correlated well with the mechanical push-out strength. Developmental age and time after injury affected the response to collagenase pretreatment. This osteochondral cartilage integration model can be useful to study factors that modulate healing of surgical replacement procedures in vitro, which may aid the development of newer approaches to promote the healing of cartilage defects.
机译：骨移植界面的整合被认为是软骨软骨移植手术中缺乏完全愈合的重要原因之一。我们开发了在骨软骨环境中软骨愈合的体外模型，以研究发育年龄和胶原酶治疗的效果。在圆柱状牛骨软骨标本的软骨部分制作了圆形的全厚度垂直手术切口。选择了两个年龄段：年轻（1-2个月大）和较大（6-8个月大）。在培养的第2和第4周，通过组织学分析和机械推出试验评估整个手术切口的软骨整合。小腿软骨的组织学整合以及峰值推剪应力显着高于小腿软骨。较老的小腿样本中的胶原酶预处理可在4周时增加推出强度。组织学整合与机械推出强度密切相关。损伤后的发育年龄和时间影响对胶原酶预处理的反应。该骨软骨软骨整合模型可用于研究调节体外手术置换过程愈合的因素，这可能有助于开发更新方法以促进软骨缺损的愈合。

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