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首页> 外文期刊>Journal of orthopaedic research >A novel injectable scaffold for cartilage tissue engineering using adipose-derived adult stem cells.
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A novel injectable scaffold for cartilage tissue engineering using adipose-derived adult stem cells.

机译：一种新型的可注射支架，用于使用脂肪来源的成体干细胞进行软骨组织工程。

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摘要

Articular cartilage has a limited self-regenerative capacity. Thus, treatment of cartilage lesions is a major challenge. Tissue engineering using a variety of biomaterials is a promising solution to the problem of cartilage damage. In this in vitro study, we investigated the effect of the presence of cartilage-tissue chondroitin-sulfate (CS) in a fibrin scaffold on the differentiation of adipose-derived adult stem cells (ADAS cells) into chondrocytes. Isolated rabbit ADAS cells were cultured in fibrin matrices with and without CS for up to 14 days. ADAS cells differentiated into chondrocytes in both matrices, but cell proliferation, glycoaminoglycans content, and type II collagen expression were significantly higher in the fibrin-CS matrices than those in the fibrin matrices alone. Histological examination and scanning electronic microscopy revealed the fibrin-CS matrices exceeded in inducing differentiation of ADAS cells into chondrocytes in terms of tissue morphological characteristics. We concluded that the fibrin-CS matrices mimicking native cartilage extracellular matrix could act as a three-dimensional scaffold for cartilage tissue engineering and have the potential for promoting ADAS cells differentiation into chondrocytes.
机译：关节软骨具有有限的自我再生能力。因此，软骨损伤的治疗是主要挑战。使用多种生物材料的组织工程是解决软骨损伤问题的有前途的解决方案。在这项体外研究中，我们研究了纤维蛋白支架中软骨组织硫酸软骨素（CS）的存在对脂肪来源的成体干细胞（ADAS细胞）分化为软骨细胞的影响。分离的兔ADAS细胞在有或没有CS的血纤蛋白基质中培养长达14天。 ADAS细胞在两种基质中均分化为软骨细胞，但在纤维蛋白-CS基质中，细胞增殖，糖胺聚糖含量和II型胶原蛋白表达明显高于单独的纤维蛋白基质。组织学检查和扫描电子显微镜显示，就组织形态学特征而言，在诱导ADAS细胞分化为软骨细胞方面超过了纤维蛋白-CS基质。我们得出的结论是，模拟天然软骨细胞外基质的纤维蛋白-CS基质可作为软骨组织工程的三维支架，并具有促进ADAS细胞分化为软骨细胞的潜力。

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