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首页> 外文期刊>Journal of orthopaedic research >Effects of heat stimulation via microwave applicator on cartilage matrix gene and HSP70 expression in the rabbit knee joint.
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Effects of heat stimulation via microwave applicator on cartilage matrix gene and HSP70 expression in the rabbit knee joint.

机译：微波辐射热刺激对兔膝关节软骨基质基因和HSP70表达的影响

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摘要

The objective of this study was to investigate the effects of heat stimulation on the expression of extracellular matrix genes and heat-shock protein 70 (HSP70) in rabbit articular cartilage in vivo. Heat stimulation was applied to the knee joints of Japanese white rabbits for 20 min using a microwave (MW) applicator (2.45-GHz, 0-80 W). After 8-72 h, the articular cartilage was removed from the knee joints and proteins and total RNA were extracted. As controls, knee joints without heat stimulation were analyzed. The expression of HSP70 was confirmed by real-time PCR and Western blotting. The expression of proteoglycan core protein (PG) and type II collagen (Col II) was quantified using real-time PCR to assess cartilage matrix metabolism. Compared to controls, HSP70 expression was higher with more than 40 W of heat stimulation. The expression of PG and Col II mRNA was higher, with more than 20 W of heat stimulation and peaked with 40 W. When quercetin was used to inhibit the induction of HSP70 expression, PG mRNA expression did not increase. External MW application stimulated HSP70 expression in the articular cartilage in vivo. The expression of extracellular matrix genes was increased by appropriate heat stimulation.
机译：本研究的目的是研究热刺激对体内兔关节软骨细胞外基质基因和热休克蛋白70（HSP70）表达的影响。使用微波（MW）施加器（2.45-GHz，0-80 W）对日本白兔的膝关节施加热刺激20分钟。 8-72小时后，从膝关节去除关节软骨，并提取蛋白质和总RNA。作为对照，分析了没有热刺激的膝关节。通过实时PCR和蛋白质印迹证实了HSP70的表达。蛋白多糖核心蛋白（PG）和II型胶原蛋白（Col II）的表达使用实时PCR定量评估软骨基质代谢。与对照相比，热刺激超过40 W时HSP70表达更高。 PG和Col II mRNA的表达较高，有超过20 W的热刺激，最高达到40W。当用槲皮素抑制HSP70表达的诱导时，PG mRNA表达没有增加。外部MW应用在体内刺激了HSP70在关节软骨中的表达。通过适当的热刺激增加细胞外基质基因的表达。

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