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首页> 外文期刊>Journal of orthopaedic research >PDGF-BB released in tendon repair using a novel delivery system promotes cell proliferation and collagen remodeling.
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PDGF-BB released in tendon repair using a novel delivery system promotes cell proliferation and collagen remodeling.

机译：使用新型递送系统在肌腱修复中释放的PDGF-BB促进细胞增殖和胶原重塑。

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摘要

The purpose of this study was to promote fibroblast proliferation and collagen remodeling in flexor tendon repair through sustained delivery of platelet derived growth factor (PDGF-BB). The release kinetics of PDGF-BB from a novel fibrin matrix delivery system was initially evaluated in vitro. After the in vivo degradation rate of the fibrin matrix was determined using fluorescently tagged fibrin, PDGF-BB was delivered to the site of flexor tendon repair in vivo in a canine model. The effect of PDGF-BB on intrasynovial tendon healing was studied using histology-based assays (cell density, proliferation, and type I collagen expression) and by measuring total DNA levels and reducible collagen crosslink levels. The fibrin matrix delivery system provided sustained release of PDGF-BB in vitro at a rate modulated by the ratio of heparin to growth factor. In vivo, the fibrin matrix remained at the repair site for more than 10 days. Delivery of PDGF-BB led to a qualitative increase in cell density, cell proliferation, and type I collagen mRNA expression. PDGF-BB also led to statistically significant increases in total DNA (20% increase at 7 days, 18% increase at 14 days) and reducible collagen crosslinks (30% increase at 7 days). Sustained delivery of growth factors may be achieved using a novel fibrin-based delivery system. PDGF-BB delivery increased cell proliferation and matrix remodeling and thus may accelerate flexor tendon healing.
机译：这项研究的目的是通过持续递送血小板衍生生长因子（PDGF-BB）促进屈肌腱修复中的成纤维细胞增殖和胶原重塑。最初在体外评估了PDGF-BB从新型纤维蛋白基质递送系统的释放动力学。在使用荧光标记的血纤蛋白测定血纤蛋白基质的体内降解速率后，PDGF-BB在犬模型中被递送至体内屈肌腱修复位点。使用基于组织学的检测方法（细胞密度，增殖和I型胶原蛋白表达）并通过测量总DNA水平和可还原的胶原蛋白交联水平，研究了PDGF-BB对滑膜内腱愈合的影响。纤维蛋白基质递送系统在体外以肝素与生长因子之比调节的速率提供了PDGF-BB的持续释放。在体内，血纤蛋白基质在修复部位保留超过10天。 PDGF-BB的交付导致细胞密度，细胞增殖和I型胶原mRNA表达的质性提高。 PDGF-BB还导致总DNA的统计学显着增加（在7天时增加20％，在14天时增加18％）和可还原的胶原交联键（在7天时增加30％）。使用新型的基于纤维蛋白的递送系统可以实现生长因子的持续递送。 PDGF-BB递送增加细胞增殖和基质重塑，因此可能加速屈肌腱愈合。

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