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首页> 外文期刊>Journal of orthopaedic research >Repair of porcine articular cartilage defect with a biphasic osteochondral composite.
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Repair of porcine articular cartilage defect with a biphasic osteochondral composite.

机译：双相骨软骨复合物修复猪关节软骨缺损。

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Autologous chondrocyte implantation (ACI) has been recently used to treat cartilage defects. Partly because of the success of mosaicplasty, a procedure that involves the implantation of native osteochondral plugs, it is of potential significance to consider the application of ACI in the form of biphasic osteochondral composites. To test the clinical applicability of such composite construct, we repaired osteochondral defect with ACI at low cell-seeding density on a biphasic scaffold, and combined graft harvest and implantation in a single surgery. We fabricated a biphasic cylindrical porous plug of DL-poly-lactide-co-glycolide, with its lower body impregnated with beta-tricalcium phosphate as the osseous phase. Osteochondral defects were surgically created at the weight-bearing surface of femoral condyles of Lee-Sung mini-pigs. Autologous chondrocytes isolated from the cartilage were seeded into the upper, chondral phase of the plug, which was inserted by press-fitting to fill the defect. Defects treated with cell-free plugs served as control. Outcome of repair was examined 6 months after surgery. In the osseous phase, the biomaterial retained in the center and cancellous bone formed in the periphery, integrating well with native subchondral bone with extensive remodeling, as depicted on X-ray roentgenography by higher radiolucency. In the chondral phase, collagen type II immunohistochemistry and Safranin O histological staining showed hyaline cartilage regeneration in the experimental group, whereas only fibrous tissue formed in the control group. On the International Cartilage Repair Society Scale, the experimental group had higher mean scores in surface, matrix, cell distribution, and cell viability than control, but was comparable with the control group in subchondral bone and mineralization. Tensile stress-relaxation behavior determined by uni-axial indentation test revealed similar creep property between the surface of the experimental specimen and native cartilage, but not the control specimen.Implanted autologous chondrocytes could survive and could yield hyaline-like cartilage in vivo in the biphasic biomaterial construct. Pre-seeding of osteogenic cells did not appear to be necessary to regenerate subchondral bone.
机译：自体软骨细胞植入（ACI）最近已用于治疗软骨缺损。部分由于镶嵌术的成功（涉及植入天然骨软骨栓的手术），考虑以双相骨软骨复合物形式应用ACI具有潜在的意义。为了测试这种复合结构的临床适用性，我们在双相支架上以低细胞播种密度用ACI修复了骨软骨缺损，并在一次手术中结合了移植物的收获和植入。我们制造了一种DL-聚丙交酯-乙交酯的双相圆柱形多孔塞，其下体浸有β-磷酸三钙作为骨相。 Lee-Sung小型猪的股骨dy负重表面通过外科手术产生了骨软骨缺损。从软骨中分离出的自体软骨细胞播种到栓的上部软骨相中，通过压入配合将其插入以填补缺损。用无细胞塞处理的缺陷作为对照。术后6个月检查修复结果。在骨质相中，生物材料保留在中心，而松质骨形成在外周，与天然软骨下骨很好地融合，并进行了广泛的重塑，如X射线放射线X线摄片所显示的那样，射线透亮性更高。在软骨期，II型胶原免疫组织化学和番红O的组织学染色显示实验组透明软骨再生，而对照组仅形成纤维组织。在国际软骨修复学会量表上，实验组在表面，基质，细胞分布和细胞活力方面的平均得分高于对照组，但在软骨下骨和矿化方面与对照组相当。通过单轴压痕测试确定的拉伸应力松弛行为表明，在实验标本的表面和天然软骨之间的蠕变特性相似，但与对照标本没有相似;植入的自体软骨细胞可以存活，并且在双相体内可以产生透明状的软骨。生物材料构造。成骨细胞的预播种似乎不是再生软骨下骨所必需的。

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