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首页> 外文期刊>Journal of orthopaedic research >Myeloperoxidase and chlorinated peptides in osteoarthritis: potential biomarkers of the disease.
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Myeloperoxidase and chlorinated peptides in osteoarthritis: potential biomarkers of the disease.

机译：骨关节炎中的髓过氧化物酶和氯化肽：该病的潜在生物标志物。

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Osteoarthritis (OA) is a disabling condition in which multiple initiating events or conditions (heritable and nonheritable) result in eventual loss of articular cartilage. However, the etiology of OA remains poorly understood, and diagnosis of early disease is difficult due to the lack of specific identifiers. Recent literature suggests that a series of inflammatory processes may be involved in initiating and propagating OA. We hypothesized that products of neutrophils and macrophages, namely myeloperoxidase (MPO), a specific enzyme responsible for the production of both highly reactive hypochlorous acid (HOCl) and chlorine gas (Cl(2)) and chlorinated peptides, may be present in the synovial fluid of patients with OA. We examined the synovial fluid from 30 patients to identify and profile the presence of MPO. We divided the samples into three groups using radiographic and clinical assessment: (1) control, patients with acute knee injury with no history of OA and no radiographic evidence of OA; (2) early OA, patients with a mild OA based on radiographs; and (3) late OA, patients with a longstanding history of OA and with radiographic evidence of complete joint loss. Patients with early OA demonstrated significantly elevated levels of MPO. We also demonstrated the presence of HOCl and Cl(2) modified proteins (Cl-peptides) in early OA synovial fluid samples by liquid chromatography and mass spectrometry. Patients in the control and advanced OA groups demonstrated little elevation in MPO levels and Cl-peptides were undetectable. These results indicate that MPO and Cl-peptides may serve as diagnostic markers for the detection of early OA.
机译：骨关节炎（OA）是一种致残性疾病，其中多种引发事件或状况（可遗传和不可遗传）导致关节软骨最终丧失。然而，OA的病因学仍知之甚少，并且由于缺乏特定的标识符而难以早期诊断疾病。最近的文献表明，一系列的炎症过程可能与OA的发生和传播有关。我们假设嗜中性粒细胞和巨噬细胞的产物，即髓过氧化物酶（MPO），一种负责产生高反应性次氯酸（HOCl）和氯气（Cl（2））和氯化肽的特定酶，可能存在于滑膜OA患者的体液。我们检查了30例患者的滑液，以鉴定和分析MPO的存在。我们通过影像学和临床评估将样本分为三组：（1）对照，无OA病史且无OA影像学证据的急性膝关节损伤患者; （2）早期OA，根据影像学检查发现轻度OA的患者; （3）晚期OA，具有悠久的OA病史且放射影像学证据表明完全丧失关节。早期OA患者表现出MPO水平明显升高。我们还通过液相色谱和质谱法证明了早期OA滑液样品中HOCl和Cl（2）修饰的蛋白（Cl肽）的存在。对照组和晚期OA组患者的MPO水平几乎没有升高，并且Cl肽无法检测到。这些结果表明，MPO和Cl肽可以作为诊断早期OA的诊断标记。

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