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首页> 外文期刊>Journal of orthopaedic research >Functional tissue engineering for tendon repair: A multidisciplinary strategy using mesenchymal stem cells, bioscaffolds, and mechanical stimulation.
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Functional tissue engineering for tendon repair: A multidisciplinary strategy using mesenchymal stem cells, bioscaffolds, and mechanical stimulation.

机译：肌腱修复的功能组织工程：使用间充质干细胞，生物支架和机械刺激的多学科策略。

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Over the past 8 years, our group has been continuously improving tendon repair using a functional tissue engineering (FTE) paradigm. This paradigm was motivated by inconsistent clinical results after tendon repair and reconstruction, and the modest biomechanical improvements we observed after repair of rabbit central patellar tendon defects using mesenchymal stem cell-gel-suture constructs. Although possessing a significantly higher stiffness and failure force than for natural healing, these first generation constructs were quite weak compared to normal tendon. Fundamental to the new FTE paradigm was the need to determine in vivo forces to which the repair tissue might be exposed. We first recorded these force patterns in two normal tendon models and then compared these peak forces to those for repairs of central defects in the rabbit patellar tendon model (PT). Replacing the suture with end-posts in culture and lowering the mesenchymal stem cell (MSC) concentration of these constructs resulted in failure forces greater than peak in vivo forces that were measured for all the studied activities. Augmenting the gel with a type I collagen sponge further increased repair stiffness and maximum force, and resulted in the repair tangent stiffness matching normal stiffness up to peak in vivo forces. Mechanically stimulating these constructs in bioreactors further enhanced repair biomechanics compared to normal. We are now optimizing components of the mechanical signal that is delivered in culture to further improve construct and repair outcome. Our contributions in the area of tendon functional tissue engineering have the potential to create functional load-bearing repairs that will revolutionize surgical reconstruction after tendon and ligament injury.
机译：在过去的8年中，我们的团队一直在使用功能组织工程（FTE）范例不断改善肌腱的修复。这种范例是由肌腱修复和重建后临床结果不一致引起的，并且在我们使用间充质干细胞-凝胶缝合结构修复兔中央tell骨肌腱缺损后观察到适度的生物力学改善。尽管与自然愈合相比，其刚度和破坏力要高得多，但与正常肌腱相比，这些第一代构造却相当脆弱。新的FTE范例的基础是需要确定修复组织可能承受的体内力。我们首先在两个正常的肌腱模型中记录这些力模式，然后将这些峰值力与修复兔tend肌腱模型（PT）中的中央缺陷的力进行比较。用培养中的末端替换缝合线并降低这些构建体的间充质干细胞（MSC）浓度会导致破坏力大于对所有研究活动测得的体内峰值力。用I型胶原海绵增强凝胶可进一步提高修复刚度和最大力，并导致修复切线刚度与正常刚度匹配，直至体内力峰值。与正常情况相比，机械刺激生物反应器中的这些构建体进一步增强了修复生物力学。现在，我们正在优化在培养中传递的机械信号的组成部分，以进一步改善构建和修复结果。我们在肌腱功能组织工程领域的贡献有潜力创造功能性的负重修复，这将彻底改变腱和韧带损伤后的手术重建。

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