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首页> 外文期刊>Journal of orthopaedic research >Activation of signal transducer and activator of transcription 3 (Stat3) pathway in osteosarcoma cells and overexpression of phosphorylated-Stat3 correlates with poor prognosis.
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Activation of signal transducer and activator of transcription 3 (Stat3) pathway in osteosarcoma cells and overexpression of phosphorylated-Stat3 correlates with poor prognosis.

机译：骨肉瘤细胞中信号转导子和转录激活子3（Stat3）通路的激活以及磷酸化Stat3的过度表达与不良预后相关。

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摘要

Stat3 expression in cancer may have important prognostic and therapeutic value, but there has been no reports correlating Stat3 expression with prognosis in patients with osteosarcoma. The goal of this study is to correlate patient prognosis with the expression of Stat3 in osteosarcoma tissue and determine the effectiveness of blocking this pathway in osteosarcoma cell lines by Stat3 inhibitor, CDDO-Me. We examine the expression levels of Stat3 and pStat3 in osteosarcoma cell lines and primary tissues by Western blot analysis. We also evaluate the levels of pStat3 expression in osteosarcoma tissue microarray (TMA) by immunohistochemistry. We use clinical data to determine the impact of levels of Stat3 expression on patient prognosis. Finally, we evaluated the effect of CDDO-Me on the inhibition of activated Stat3 pathway in osteosarcoma cell lines using MTT assay and Western blot analysis. Stat3 is observed to be activated in osteosarcoma tissues as well as in cultured cell lines. Overexpression of pStat3 is associated with poor prognosis. CDDO-Me inhibits the growth of osteosarcoma cell lines and induces apoptosis as well. Our results suggest that Stat3 may be a prognostic indicator and potential therapeutic target for osteosarcoma. Blocking the pathway of Stat3 may lead to develop new therapeutic strategies against osteosarcoma.
机译：Stat3在癌症中的表达可能具有重要的预后和治疗价值，但尚无有关Stat3表达与骨肉瘤患者预后相关的报道。这项研究的目的是使患者的预后与Stat3在骨肉瘤组织中的表达相关，并确定通过Stat3抑制剂CDDO-Me阻断骨肉瘤细胞系中该途径的有效性。我们通过蛋白质印迹分析检查了骨肉瘤细胞系和主要组织中Stat3和pStat3的表达水平。我们还通过免疫组织化学评估了骨肉瘤组织微阵列（TMA）中pStat3表达的水平。我们使用临床数据确定Stat3表达水平对患者预后的影响。最后，我们使用MTT分析和Western印迹分析评估了CDDO-Me对骨肉瘤细胞系中激活的Stat3途径的抑制作用。 Stat3被观察到在骨肉瘤组织以及培养的细胞系中被激活。 pStat3的过表达与预后不良有关。 CDDO-Me抑制骨肉瘤细胞系的生长并诱导凋亡。我们的结果表明，Stat3可能是骨肉瘤的预后指标和潜在治疗靶标。阻断Stat3的途径可能导致开发针对骨肉瘤的新治疗策略。

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