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首页> 外文期刊>Journal of orthopaedic research >Rapid attachment of adipose stromal cells on resorbable polymeric scaffolds facilitates the one-step surgical procedure for cartilage and bone tissue engineering purposes.
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Rapid attachment of adipose stromal cells on resorbable polymeric scaffolds facilitates the one-step surgical procedure for cartilage and bone tissue engineering purposes.

机译：脂肪基质细胞在可吸收的聚合物支架上的快速附着促进了用于软骨和骨组织工程目的的一步式手术程序。

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The stromal vascular fraction (SVF) of adipose tissue provides an abundant source of mesenchymal stem cells. For clinical application, it would be beneficial to establish treatments in which SVF is obtained, seeded onto a scaffold, and returned into the patient within a single surgical procedure. In this study, we evaluated the suitability of both a macroporous poly(L-lactide-co-caprolactone) and a porous collagen type I/III scaffold for this purpose. Surprisingly, cell attachment was rapid ( approximately 10 min) and sequestered the majority of adipose stem cells, as deduced from colony-forming unit assays. Proliferation occurred in both polymeric scaffolds. Upon chondrogenic induction, up-regulation of chondrogenic genes, production of glycosaminoglycans, and accumulation of collagen type II was observed, indicating differentiation of scaffold-attached SVF cells along the chondrogenic lineage. Osteogenic differentiation was achieved in both scaffold types, as visualized by up-regulation of osteogenic genes, increase of alkaline phosphatase production over time, and accumulation of bone sialoprotein and osteonectin. In conclusion, this study identifies both poly(L-lactide-co-caprolactone) and collagen type I/III as promising scaffold materials for rapid attachment of adipose stem cell-like (stromal) cells, enhancing the development of one-step surgical concepts for cartilage and bone tissue engineering.
机译：脂肪组织的基质血管部分（SVF）提供了间充质干细胞的丰富来源。对于临床应用而言，建立一种在单个手术过程中获得SVF，接种到支架上并返回患者的治疗方法将是有益的。在这项研究中，我们评估了大孔聚（L-丙交酯-己内酯）和多孔I / III型胶原蛋白支架的适用性。出人意料的是，细胞附着迅速（约10分钟），并隔离了大多数的脂肪干细胞，这是从菌落形成单位测定法得出的。两种聚合物支架均发生增殖。在软骨形成诱导后，观察到软骨生成基因的上调，糖胺聚糖的产生和II型胶原的积累，表明沿软骨生成谱系的支架附着的SVF细胞分化。通过成骨基因的上调，随着时间的推移碱性磷酸酶产生的增加以及骨唾液蛋白和骨连接蛋白的积累，可以看出两种支架都可以实现成骨分化。总之，这项研究确定了聚（L-丙交酯-己内酯）和I / III型胶原是有希望的支架材料，可快速附着于脂肪干细胞样（基质）细胞，从而促进了一步式外科手术概念的发展用于软骨和骨组织工程。

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