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首页> 外文期刊>Journal of orthopaedic research >Polymethylmethacrylate and titanium alloy particles activate peripheral monocytes during periprosthetic inflammation and osteolysis.
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Polymethylmethacrylate and titanium alloy particles activate peripheral monocytes during periprosthetic inflammation and osteolysis.

机译：在假体周围炎症和溶骨过程中，聚甲基丙烯酸甲酯和钛合金颗粒激活外周单核细胞。

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We investigated the interactions of particulate PMMA or titanium alloy, patient blood monocytes, and periprosthetic tissues using a SCID-hu model of aseptic loosening. Periprosthetic tissues and bone chips obtained at revision surgery for loosening were transplanted into muscles of SCID mice. Peripheral blood monocytes (PBMCs) isolated from the same donors were fluorescently labeled and co-cultured with PMMA or Ti-6Al-4V particles before intraperitoneal injection. Control mice with periprosthetic tissue or non-inflammatory ligament xenografts received naive PBMCs transfusion. Mice were euthanized 2 weeks after PBMC transfusion. The human tissues were well accepted in SCID mice. Transfused fluorescent-labeled PBMCs were markedly accumulated in transplanted periprosthetic tissues. Multinucleated osteoclast-like cells were commonly seen within retrieved xenograft tissue, and focal bone erosions were ubiquitous. Total cell densities and CD68+ cells within the xenograft were significantly increased in mice transfused with PMMA and Ti-provoked PBMCs compared to the naive PBMC animals (p < 0.05). Immunohistochemical staining identified much stronger positive IL-1 and TNF stains in xenografts from either PMMA or Ti-stimulated monocytes transfusion groups (p < 0.05). TRAP+ cells were found around bone chips in both activated-PBMCs groups, although markedly more aggregated TRAP+ cells in the PMMA-challenged group than in the titanium group (p < 0.05). MicroCT assessment confirmed the significant decrease of bone mineral density in chips interacted with activated-monocytes/osteoclasts. In conclusion, PMMA or titanium particles readily activate peripheral monocytes and promote the cell trafficking to the debris-containing prosthetic tissues. Particles-provoked PBMCs participated in and promoted the local inflammatory process, osteoclastogenesis, and bone resorption.
机译：我们使用无菌松动的SCID-hu模型研究了颗粒状PMMA或钛合金，患者血液单核细胞和假体周围组织的相互作用。在翻修手术中获得的用于松动的骨膜周围组织和骨屑被移植到SCID小鼠的肌肉中。对从相同供体分离的外周血单核细胞（PBMC）进行荧光标记，并在腹膜内注射之前与PMMA或Ti-6Al-4V颗粒共培养。具有假体周围组织或非炎性韧带异种移植物的对照小鼠接受幼稚PBMC输血。 PBMC输注2周后对小鼠实施安乐死。在SCID小鼠中人体组织被很好地接受。输注的荧光标记的PBMC在移植的假体周围组织中明显积累。在取出的异种移植组织中通常见到多核破骨细胞样细胞，并且局灶性骨侵蚀普遍存在。与未使用过PBMC的动物相比，在用PMMA和Ti诱导的PBMC输注的小鼠中，异种移植物中的总细胞密度和CD68 +细胞显着增加（p <0.05）。免疫组织化学染色在来自PMMA或Ti刺激的单核细胞输血组的异种移植物中鉴定出更强的阳性IL-1和TNF染色（p <0.05）。在两个激活的PBMCs组中，都在骨碎片周围发现了TRAP +细胞，尽管在PMMA挑战组中，聚集的TRAP +细胞明显多于钛组（p <0.05）。 MicroCT评估证实与活化单核细胞/破骨细胞相互作用的芯片中骨矿物质密度显着降低。总之，PMMA或钛颗粒很容易激活外周单核细胞并促进细胞向含有碎屑的假体组织的运输。颗粒诱发的PBMC参与并促进了局部炎症过程，破骨细胞生成和骨吸收。

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