...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of orthopaedic research >Effect of chondroitinase ABC on adhesion and behavior of synovial membrane-derived mesenchymal stem cells in rabbit partial-thickness chondral defects
【24h】

Effect of chondroitinase ABC on adhesion and behavior of synovial membrane-derived mesenchymal stem cells in rabbit partial-thickness chondral defects

机译：软骨素酶ABC对滑膜膜间充质干细胞在兔部分厚度软骨缺损中粘附和行为的影响

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Transplanted cells may have difficulty attaching to the surface of partial-thickness chondral lesions because of the anti-adhesive properties of the proteoglycan rich matrix. Therefore, the current study attempts to evaluate the effect of chondroitinase ABC (chABC) on the adhesion and behavior of transplanted synovial membrane-derived mesenchymal stem cells (SDSCs) in rabbit partial-thickness chondral defects. In ex vivo adhesion experiments, chABC treatment (0.1 U/ml) was increased in SDSC attachment to the cartilage explants, and significantly diminished by pretreatment with neutralizing antibody against fibronectin. In the in vivo experiments, 1 day and 4 weeks after the chABC treatment (0.1 and 1 U/ml), the immunoreactivity (IR) against CS-56 (intact chondroitin sulfate antibody) was markedly decreased; however, the IR of 2B6 (stub of the chondroitin 4-sulfate chain), 3B3 (stub of the chondroitin 6-sulfate chain), and fibronectin was increased. At 12 weeks, this IR returned to normal except in the high-dose chABC-treated group (1 U/ml). Furthermore, the attachment of SDSCs to the chondral defects after chABC treatment was increased at 7 days compared with that in the chondral defects pretreated with saline. However, the tissue repaired by SDSCs was negatively stained for type II collagen at 12 weeks. In conclusion, these results showed that the exposure to fibronectin by chABC treatment enhances the attachment of SDSCs to partial-thickness chondral defects. However, the tissue regenerated by SDSCs showed lack of hyaline cartilage regeneration. Thus, to understand the fate of transplanted MSCs in cartilage defect is very important for successful cell therapies.
机译：由于富含蛋白多糖的基质的抗粘附特性，移植的细胞可能难以附着到部分厚度的软骨损伤表面。因此，当前的研究试图评估软骨素酶ABC（chABC）对兔滑膜软骨间质干细胞（SDSCs）黏附和行为的影响。在离体粘附实验中，chABC处理（0.1 U / ml）在SDSC与软骨外植体的附着中增加，并通过用抗纤连蛋白的中和抗体进行预处理而大大降低。在体内实验中，chABC处理后第1天和第4周（0.1和1 U / ml），针对CS-56（完整的硫酸软骨素抗体）的免疫反应性（IR）明显降低;但是，2B6（软骨素4硫酸盐链的残基），3B3（软骨素6硫酸盐链的残基）和纤连蛋白的IR增加。在第12周时，该IR恢复正常，除了在高剂量chABC治疗组（1 U / ml）中。此外，与用盐水预处理的软骨缺损相比，在chABC处理后SDSCs在软骨缺损处的附着增加了7天。然而，由SDSC修复的组织在12周时对II型胶原蛋白进行了负染色。总之，这些结果表明，通过chABC处理暴露于纤连蛋白会增强SDSC与部分厚度的软骨缺损的附着。然而，由SDSCs再生的组织显示缺乏透明软骨再生。因此，了解移植的MSC在软骨缺损中的命运对于成功的细胞治疗非常重要。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号