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首页> 外文期刊>Journal of orthopaedic research >ADAMTS-5 and intervertebral disc degeneration: the results of tissue immunohistochemistry and in vitro cell culture.
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ADAMTS-5 and intervertebral disc degeneration: the results of tissue immunohistochemistry and in vitro cell culture.

机译：ADAMTS-5和椎间盘退变：组织免疫组织化学和体外细胞培养的结果。

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Matrix metalloproteinases (MMPs) are known to be involved in IVD degeneration by hydrolyzing the extracellular matrix (ECM), especially the collagens. The degradation of proteoglycans, which is another main ECM component in the IVD, however, has not been extensively investigated. This study aimed to determine the expression of ADAMTS-5 in human herniated intervertebral disc (IVD) tissues and to investigate whether interleukin-1beta (IL-1beta)-induced expression of ADAMTS-5 is mediated by nitric oxide (NO). Forty-five herniated IVDs were harvested and immunostained to determine the distribution and type of ADAMTS-5 expressing cells. Rat NP cells maintained in alginate beads were treated with IL-1beta, accumulation of NO was detected by Griess reaction, the expression of ADAMTS-5 and inducible nitric oxide synthase (iNOS) was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR), the content of proteoglycans in alginate beads was visualized by alcian blue staining, and the effect of aminoguanidine on the changes in alginate beads induced by IL-1beta treatment were also examined. Immunohistochemical results from 45 herniated discs showed that ADAMTS-5-positive cells are commonly seen in cell clusters, that the percentage of ADAMTS-5-positive cells was higher in uncontained herniated discs than in contained ones, and that the percentage of ADAMTS-5-positive cells correlated with the age of the patients. IL-1beta treatment resulted in increased accumulation of NO, increased expression of ADAMTS-5 and iNOS, whereas the accumulation of proteoglycan in alginate beads decreased. Aminoguanidine significantly reversed the changes in alginate beads induced by IL-1beta treatment. We thus suggested that ADAMTS-5 is probably involved in the process of IVD degeneration, and that IL-1beta-induced expression of ADAMTS-5 is mediated by NO.
机译：已知基质金属蛋白酶（MMP）通过水解细胞外基质（ECM）特别是胶原蛋白参与IVD变性。蛋白多糖的降解是IVD中另一个主要的ECM成分，但是尚未得到广泛研究。这项研究旨在确定ADAMTS-5在人椎间盘（IVD）组织中的表达，并调查白介素1beta（IL-1beta）诱导的ADAMTS-5表达是否由一氧化氮（NO）介导。收获四十五个突出的IVD并进行免疫染色以确定表达ADAMTS-5的细胞的分布和类型。用IL-1beta处理维持在藻酸盐珠中的大鼠NP细胞，通过Griess反应检测NO的积累，通过逆转录聚合酶链反应（RT-PCR）分析ADAMTS-5和诱导型一氧化氮合酶（iNOS）的表达。 ），通过阿尔辛蓝染色观察藻酸盐珠中蛋白聚糖的含量，并检查了氨基胍对IL-1β处理诱导的藻酸盐珠变化的影响。来自45个椎间盘的免疫组织化学结果显示，ADAMTS-5阳性细胞常见于细胞簇中，未携带的椎间盘中ADAMTS-5阳性细胞的百分比高于包含的椎间盘，ADAMTS-5的百分比阳性细胞与患者年龄有关。 IL-1beta处理导致NO积累增加，ADAMTS-5和iNOS表达增加，而藻酸盐珠中蛋白聚糖的积累减少。氨基胍显着逆转了由IL-1β处理诱导的藻酸盐珠的变化。因此，我们建议ADAMTS-5可能与IVD变性有关，而IL-1β诱导的ADAMTS-5表达是由NO介导的。

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