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首页> 外文期刊>Journal of orthopaedic research >Synergy of tendon stem cells and platelet-rich plasma in tendon healing
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Synergy of tendon stem cells and platelet-rich plasma in tendon healing

机译：肌腱干细胞与富血小板血浆在肌腱愈合中的协同作用

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Injured rat Achilles tendons were treated with botulism toxin to create a mechanically unloaded condition (unloaded) or left untreated (loaded), and then treated with phosphate-buffered saline (PBS), platelet-rich plasma (PRP), tendon stem cells (TSCs), or a combination (TSCs + PRP). mRNA and protein expression of collagen I, collagen III, tenascin C, and Smad 8 were determined by real time PCR and immunostaining, respectively. Loaded tendons treated with PBS, PRP, or TSCs for 3 or 14 days had higher collagen I mRNA expression than unloaded tendons. Loaded tendons treated with PBS for 3 or 14 days or with PRP for 3 days had higher collagen I protein levels than unloaded tendons. Loaded tendons treated for 3 days with PBS, for 14 days with PRP or TSCs or TSCs + PRP for 3 or 14 days had higher collagen III protein levels than unloaded tendons. Collagen I mRNA levels were higher in TSCs + PRP-treated loaded tendons compared to PBS-treated loaded tendons on day 3 of treatment. Based on changes in the expression of tendon-healing genes, our data suggest that the combination of TSCs and PRP has synergistic effects on tendon healing under both loaded and unloaded conditions, and loaded conditions improve tendon healing.
机译：用肉毒杆菌毒素处理受伤的大鼠跟腱以产生机械负荷状态（未负荷）或不进行处理（负荷），然后用磷酸盐缓冲液（PBS），富含血小板的血浆（PRP），腱干细胞（TSC）处理）或组合（TSC + PRP）。通过实时PCR和免疫染色分别测定胶原蛋白I，胶原蛋白III，腱糖蛋白C和Smad 8的mRNA和蛋白表达。用PBS，PRP或TSC处理3或14天的肌腱比未肌腱具有更高的I型胶原mRNA表达。用PBS处理3或14天或用PRP处理3天的肌腱的胶原I蛋白水平要高于未负载的肌腱。用PBS处理3天，用PRP或TSCs或TSCs + PRP处理3天或14天的肌腱比未负载的肌腱具有更高的胶原蛋白III蛋白水平。在治疗的第3天，与经PBS处理的负载肌腱相比，经TSCs + PRP处理的负载肌腱中胶原I mRNA水平更高。基于肌腱愈合基因表达的变化，我们的数据表明TSC和PRP的组合在有载和无载条件下均对肌腱愈合具有协同作用，而有载条件可改善肌腱愈合。

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