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首页> 外文期刊>Journal of orthopaedic research >Effects of lactic acid and glycolic acid on human osteoblasts: A way to understand PLGA involvement in PLGA/calcium phosphate composite failure
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Effects of lactic acid and glycolic acid on human osteoblasts: A way to understand PLGA involvement in PLGA/calcium phosphate composite failure

机译：乳酸和乙醇酸对人成骨细胞的影响：了解PLGA参与PLGA /磷酸钙复合材料衰竭的一种方法

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摘要

The use of degradable composite materials in orthopedics remains a field of intense research due to their ability to support new bone formation and degrade in a controlled manner, broadening their use for orthopedic applications. Poly (lactide-co-glycolide) acid (PLGA), a degradable biopolymer, is now a popular material for different orthopedic applications and is proposed for use in tissue engineering scaffolds either alone or combined with bioactive ceramics. Interference screws composed of calcium phosphates and PLGA are readily available in the market. However, some reports highlight problems of screw migration or aseptic cyst formation following screw degradation. In order to understand these phenomena and to help to improve implant formulation, we have evaluated the effects of PLGA degradation products: lactic acid and glycolic acid on human osteoblasts in vitro. Cell proliferation, differentiation, and matrix mineralization, important for bone healing were studied. It was found that the toxicity of polymer degradation products under buffering conditions was limited to high concentrations. However, non-toxic concentrations led to a decrease in cell proliferation, rapid cell differentiation, and mineralization failure. Calcium, whilst stimulating cell proliferation was not able to overcome the negative effects of high concentrations of lactic and glycolic acids on osteoblasts. These effects help to explain recently reported clinical failures of calcium phosphate/PLGA composites, but further in vitro analyses are needed to mimic the dynamic situation which occurs in the body by, for example, culture of osteoblasts with materials that have been pre-degraded to different extents and thus be able to relate these findings to the degradation studies that have been performed previously.
机译：由于可降解复合材料支持新骨形成并以受控方式降解的能力，因此在整形外科中仍使用可降解复合材料，从而扩大了其在整形外科领域的应用范围。聚（丙交酯-共-乙交酯）酸（PLGA）是一种可降解的生物聚合物，现已成为各种骨科应用的流行材料，并被提议单独或与生物活性陶瓷结合用于组织工程支架。由磷酸钙和PLGA组成的干涉螺钉可在市场上买到。然而，一些报道突出了螺钉降解后螺钉迁移或无菌性囊肿形成的问题。为了了解这些现象并帮助改进植入物配方，我们评估了PLGA降解产物：乳酸和乙醇酸对人成骨细胞的体外作用。研究了细胞增殖，分化和基质矿化对骨骼愈合至关重要。发现在缓冲条件下聚合物降解产物的毒性限于高浓度。但是，无毒浓度导致细胞增殖减少，细胞快速分化和矿化失败。钙虽然能刺激细胞增殖，却不能克服高浓度的乳酸和乙醇酸对成骨细胞的不利影响。这些作用有助于解释最近报道的磷酸钙/ PLGA复合材料的临床失败，但是需要进一步的体外分析来模拟体内发生的动态情况，例如，通过使用已经预先降解为程度不同，因此能够将这些发现与先前进行的降解研究相关联。

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