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首页> 外文期刊>Journal of orthopaedic research >Human mesenchymal stem cells in synovial fluid increase in the knee with degenerated cartilage and osteoarthritis
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Human mesenchymal stem cells in synovial fluid increase in the knee with degenerated cartilage and osteoarthritis

机译：膝关节滑膜液中的人间充质干细胞在软骨变性和骨关节炎中增加

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摘要

We investigated whether mesenchymal stem cells (MSCs) in synovial fluid (SF) increased in the knee with degenerated cartilage and osteoarthritis. SF was obtained from the knee joints of 22 patients with anterior cruciate ligament (ACL) injury during ACL reconstruction, and cartilage degeneration was evaluated arthroscopically. SF was also obtained from the knee joints of 6 healthy volunteers, 20 patients with mild osteoarthritis, and 26 patients with severe osteoarthritis, in which the grading was evaluated radiographically. The cell component in the SF was cultured for analyses. Synovium (SYN) and bone marrow (BM) were also harvested during total knee arthroplasties. The MSC number in SF was correlated with the cartilage degeneration score evaluated by arthroscopy. The MSC number in the SF was hardly noticed in normal volunteers, but it increased in accordance with the grading of osteoarthritis. Though no significant differences were observed regarding surface epitopes, or differentiation potentials, the morphology and gene profiles in SF MSCs were more similar to those in SYN MSCs than in BM MSCs. We listed 20 genes which were expressed higher in both SYN MSCs and SF MSCs than in BM MSCs, and 3 genes were confirmed by quantitative RT-PCR. MSCs in SF increased along with degenerated cartilage and osteoarthritis.
机译：我们调查了滑膜液（SF）中的间充质干细胞（MSC）是否在变性软骨和骨关节炎的情况下在膝盖中增加。从22例ACL重建过程中前交叉韧带（ACL）损伤的膝关节中获得SF，并通过关节镜评估软骨变性。还从6名健康志愿者的膝关节，20例轻度骨关节炎和26例重度骨关节炎的患者的膝关节中获得了SF，通过放射线照相评估了分级。培养SF中的细胞成分以进行分析。在全膝关节置换术中也收获了滑膜（SYN）和骨髓（BM）。 SF中的MSC数目与通过关节镜评估的软骨变性评分相关。在正常志愿者中几乎没有发现SF中的MSC数量，但随着骨关节炎等级的增加而增加。尽管在表面表位或分化潜能上没有观察到显着差异，但SF MSC的形态和基因谱与SYN MSC相比，与BM MSC更为相似。我们列出了20个在SYN MSC和SF MSC中均比在BM MSC中表达的基因，并且通过定量RT-PCR证实了3个基因。 SF中的MSC伴随变性软骨和骨关节炎而增加。

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