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首页> 外文期刊>Journal of orthopaedic research >Wnt/-catenin signaling of cartilage canal and osteochondral junction chondrocytes and full thickness cartilage in early equine osteochondrosis
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Wnt/-catenin signaling of cartilage canal and osteochondral junction chondrocytes and full thickness cartilage in early equine osteochondrosis

机译：马骨骼骨软骨病中软骨管和骨软骨连接软骨细胞和全层软骨的Wnt / catenin信号传导

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The objective of this study was to elucidate gene and protein expression of Wnt signaling molecules in chondrocytes of foals having early osteochondrosis (OC) versus normal controls. The hypothesis was that increased expression of components of Wnt signaling pathway in osteochondral junction (OCJ) and cartilage canal (CC) chondrocytes would be found in early OC when compared to controls. Paraffin-embedded osteochondral samples (7 OC, 8 normal) and cDNA from whole cartilage (7 OC, 10 normal) and chondrocytes surrounding cartilage canals and osteochondral junctions captured with laser capture microdissection (4 OC, 6 normal) were obtained from femoropatellar joints of 17 immature horses. Equine-specific Wnt signaling molecule mRNA expression levels were evaluated by two-step real-time qPCR. Spatial tissue protein expression of -catenin, Wnt-11, Wnt-4, and Dkk-1 was determined by immunohistochemistry. There was significantly decreased Wnt-11 and increased -catenin, Wnt-5b, Dkk-1, Lrp6, Wif-1, Axin1, and SC-PEP gene expression in early OC cartilage canal chondrocytes compared to controls. There was also significantly increased -catenin gene expression in early OC osteochondral junction chondrocytes compared to controls. Based on this study, abundant gene expression differences in OC chondrocytes surrounding cartilage canals suggest pathways associated with catabolism and inhibition of chondrocyte maturation are targeted in early OC pathogenesis. (c) 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:1433-1438, 2015.
机译：这项研究的目的是阐明与正常对照组相比，患有早期骨软骨病（OC）的小马的软骨细胞中Wnt信号分子的基因和蛋白质表达。假说是，与对照组相比，早期OC会发现Wnt信号通路在骨软骨连接（OCJ）和软骨管（CC）软骨细胞中的表达增加。石蜡包埋的骨软骨样品（7 OC，8正常）和来自整个软骨的cDNA（7 OC，10正常）以及通过激光捕获显微切割术捕获的软骨和软骨周围结膜的软骨细胞（4 OC，6正常）取自股骨of关节17匹未成熟的马。马特异性Wnt​​信号分子mRNA表达水平通过两步实时qPCR进行评估。通过免疫组织化学确定-catenin，Wnt-11，Wnt-4和Dkk-1的空间组织蛋白表达。与对照组相比，早期OC软骨管软骨细胞中的Wnt-11明显减少，而-catenin，Wnt-5b，Dkk-1，Lrp6，Wif-1，Axin1和SC-PEP基因表达明显增加。与对照相比，早期OC骨软骨连接软骨细胞中-catenin基因表达也显着增加。根据这项研究，软骨管周围的OC软骨细胞中大量的基因表达差异表明与分解代谢相关的途径和对软骨细胞成熟的抑制是早期OC发病机理的目标。 （c）2015骨科研究学会。由Wiley Periodicals，Inc.出版，J Orthop Res 33：1433-1438，2015。

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