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首页> 外文期刊>Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis >Simultaneous determination of danshensu, rosmarinic acid, cryptotanshinone, tanshinone IIA, tanshinone I and dihydrotanshinone I by liquid chromatographic-mass spectrometry and the application to pharmacokinetics in rats.
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Simultaneous determination of danshensu, rosmarinic acid, cryptotanshinone, tanshinone IIA, tanshinone I and dihydrotanshinone I by liquid chromatographic-mass spectrometry and the application to pharmacokinetics in rats.

机译：液相色谱-质谱法同时测定丹参素，迷迭香酸，隐丹参酮，丹参酮IIA，丹参酮I和二氢丹参酮I，并将其应用于大鼠药代动力学。

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摘要

A sensitive and specific liquid chromatography-tandem mass spectrometry method (LC-MS) was developed and validated for the separation and simultaneous determination of danshensu, rosmarinic acid and tanshinone compounds including cryptotanshinone, tanshinone I, dihydrotanshinone I and tanshinone IIA in rat plasma. Chromatographic separation of the analytes was successfully achieved on a C(18) column using a mobile phase composed of acetonitrile-water containing 0.5% glacial acetic acid. This method demonstrated good linearity and did not have endogenous material interfering with the active compounds and I.S. peaks. The limit of quantification of danshensu, rosmarinic acid, cryptotanshinone, dihydrotanshinone I, tanshinone I and tanshinone IIA were 5, 0.75, 0.1, 0.1, 1 and 0.5 ng/mL. The average extraction recoveries of these analytes from rat plasma were all over 60%. The precisions determined from five days were all within 10%. This method has been successfully applied in the simultaneous quantification and the pharmacokinetic studies of these six compounds in animals which were orally administered with danshen preparations.
机译：建立了灵敏且特异性的液相色谱-串联质谱法（LC-MS），并验证了大鼠血浆中丹参素，迷迭香酸和丹参酮化合物（包括隐丹参酮，丹参酮I，二氢丹参酮I和丹参酮IIA）的分离和同时测定的有效性。使用包含0.5％冰醋酸的乙腈-水组成的流动相，在C（18）色谱柱上成功实现了分析物的色谱分离。该方法显示出良好的线性，并且没有干扰活性化合物和I.S.的内源性物质。高峰。丹参素，迷迭香酸，隐丹参酮，二氢丹参酮I，丹参酮I和丹参酮IIA的定量限为5、0.75、0.1、0.1、1和0.5 ng / mL。这些分析物从大鼠血浆中的平均提取回收率均超过60％。五天后确定的精度都在10％以内。该方法已成功应用于这六种化合物在丹参制剂口服给药的动物的同时定量和药代动力学研究中。

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