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首页> 外文期刊>Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis >A chemical profiling strategy for semi-quantitative analysis of flavonoids in Ginkgo extracts
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A chemical profiling strategy for semi-quantitative analysis of flavonoids in Ginkgo extracts

机译：半定量分析银杏提取物中黄酮类化合物的化学分析策略

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摘要

Flavonoids analysis in herbal products is challenged by their vast chemical diversity. This work aimed to develop a chemical profiling strategy for the semi-quantification of flavonoids using extracts of Ginkgo biloba L. (EGB) as an example. The strategy was based on the principle that flavonoids in EGB have an almost equivalent molecular absorption coefficient at a fixed wavelength. As a result, the molecular contents of flavonoids were able to be semi-quantitatively determined by the molecular-concentration calibration curves of common standards and recalculated as the mass-contents with the characterized molecular weight (MW). Twenty batches of EGB were subjected to HPLC-UV/DAD/MS fingerprinting analysis to test the feasibility and reliability of this strategy. The flavonoid peaks were distinguished from the other peaks with principle component analysis and Pearson correlation analysis of the normalized UV spectrometric dataset. Each flavonoid peak was subsequently tentatively identified by the MS data to ascertain their MW. It was highlighted that the flavonoids absorption at Band-II (240-280 nm) was more suitable for the semi-quantification purpose because of the less variation compared to that at Band-I (300-380 nm). The semi-quantification was therefore conducted at 254 nm. Beyond the qualitative comparison results acquired by common chemical profiling techniques, the semi-quantitative approach presented the detailed compositional information of flavonoids in EGB and demonstrated how the adulteration of one batch was achieved. The developed strategy was believed to be useful for the advanced analysis of herbal extracts with a high flavonoid content without laborious identification and isolation of individual components. (C) 2016 Elsevier B.V. All rights reserved.
机译：草药产品中的类黄酮分析面临着巨大的化学多样性挑战。这项工作旨在以银杏叶提取物（EGB）为例，开发一种用于半黄酮类半定量的化学分析策略。该策略基于以下原理：EGB中的类黄酮在固定波长下具有几乎相等的分子吸收系数。结果，类黄酮的分子含量可以通过通用标准品的分子浓度校准曲线进行半定量测定，并重新计算为具有特征分子量（MW）的质量含量。对20批EGB进行了HPLC-UV / DAD / MS指纹分析，以检验该策略的可行性和可靠性。通过归一化UV光谱数据集的主成分分析和Pearson相关分析，可将类黄酮峰与其他峰区分开。随后通过MS数据初步鉴定出每个类黄酮峰，以确定其分子量。强调指出，由于与Band-I（300-380 nm）相比，变化较小，Band-II（240-280 nm）处的类黄酮吸收更适合于半定量分析。因此，半定量在254nm下进行。除了通过常规化学分析技术获得的定性比较结果之外，半定量方法还提供了EGB中类黄酮的详细成分信息，并演示了如何实现一批掺假。据信，所开发的策略可用于高级分析具有高黄酮含量的草药提取物，而无需费力地鉴定和分离各个成分。 （C）2016 Elsevier B.V.保留所有权利。

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