• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis >Comparison of extraction of a beta-blocker from plasma onto a molecularly imprinted polymer with liquid-liquid extraction and solid phase extraction methods.
【24h】

Comparison of extraction of a beta-blocker from plasma onto a molecularly imprinted polymer with liquid-liquid extraction and solid phase extraction methods.

机译：使用液-液萃取和固相萃取方法比较从血浆中提取β受体阻滞剂到分子印迹聚合物上。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An optimised solid phase extraction (SPE) method developed for the extraction of a structural analogue of the beta-blocking drug propranolol from plasma utilising a molecularly imprinted polymer (MIP) has been compared with methods based on conventional liquid-liquid extraction (LLE), and SPE using C18-bonded and immobilised phenyl boronic acid (PBA). All four methods could be used for the extraction of the analyte with acceptable accuracy and precision. The MIP-based method, unlike the other methods required a protein precipitation step prior to extraction to eliminate the effects of co-extracted protein. The best performance was seen with the LLE method followed by SPE on the C18 phase. The MIP-based method represented no advantage over the comparator methods for this analyte. Indeed the performance of the MIP-based method was marginally worse as leaching of low level template impurities prevented detection of the target analyte at low concentrations (5ngmL(-1)). This relatively poorer performance was evident as worse accuracy at low concentrations with a consequent higher limit of quantification than the conventional methods.
机译：已开发出一种利用分子印迹聚合物（MIP）从血浆中提取β受体阻断剂普萘洛尔的结构类似物的优化固相萃取（SPE）方法与基于常规液液萃取（LLE）的方法进行了比较，以及使用C18键合并固定的苯基硼酸（PBA）的SPE。所有这四种方法都可以以可接受的精度和精确度用于分析物的提取。与其他方法不同，基于MIP的方法需要在提取前进行蛋白质沉淀步骤，以消除共提取蛋白质的作用。用LLE方法观察到最佳性能，然后在C18相上进行SPE。对于这种分析物，基于MIP的方法与比较器方法相比没有优势。实际上，基于MIP的方法的性能稍差，因为低水平模板杂质的浸出阻止了低浓度（5ngmL（-1））目标分析物的检测。这种相对较差的性能很明显，因为在低浓度下精度较差，因此定量限比常规方法更高。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号