• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis >Detection methods of microsphere based single-step bioaffinity and in vitro diagnostics assays.
【24h】

Detection methods of microsphere based single-step bioaffinity and in vitro diagnostics assays.

机译：基于微球的一步生物亲和力检测方法和体外诊断测定。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Microspheres provide a solid phase substrate for bioaffinity binding similar to the walls of traditional test tubes and the wells of microtiter plates. The coated microsphere concentrates analyte molecules in the reaction volume on its surface. When the bioaffinity binding reaction has reached an equilibrium, the local concentration of the analyte in close proximity of the microsphere is orders of magnitude higher than the concentration of the analyte in the total reaction volume. The preparation and quality control of microspheres coated with bioactive material is less costly and labour intensive when compared to test tube or microwell plate coating procedures. In addition, the cost for logistics and transportation of microsphere reagents is lower than that of coated tubes or plates. Moreover, microspheres can be easily used in miniaturised assay formats and several different detection schemes can be employed in the measurement of microsphere-based assays. Several different types of microspheres are commercially available. The microspheres can be manufactured in different sizes from many materials, such as polystyrene, acrylate, and glass. The surface of the microspheres can be activated to enable covalent binding of biomolecules. Further, the microspheres may contain internal fluorochrome or magnetic material, for identification or separation purposes. In this paper we review different assay formats for single-step measurement of bioaffinity assays employing microspheres. The term single-step is used to describe assays where all reagents and the sample are mixed, incubated and measured without separate washing steps.
机译：微球提供了用于生物亲和力结合的固相底物，类似于传统试管的壁和微量滴定板的孔。包被的微球将分析物分子浓缩在其表面的反应体积中。当生物亲和力结合反应达到平衡时，在微球附近的分析物的局部浓度要比总反应体积中的分析物的浓度高几个数量级。与试管或微孔板的涂覆程序相比，用生物活性材料涂覆的微球的制备和质量控制成本更低，劳动强度更高。另外，微球试剂的物流和运输成本低于涂覆管或板的成本。而且，微球可以容易地以小型化的测定形式使用，并且在基于微球的测定的测量中可以采用几种不同的检测方案。几种不同类型的微球是可商购的。可以用多种材料，例如聚苯乙烯，丙烯酸酯和玻璃，以不同的尺寸制造微球。可以活化微球的表面以使生物分子共价结合。此外，微球可包含内部荧光染料或磁性材料，以用于识别或分离目的。在本文中，我们回顾了采用微球进行生物亲和力测定的单步测量的不同测定形式。术语“单步”用于描述无需单独洗涤步骤即可混合，孵育和测量所有试剂和样品的分析方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号