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首页> 外文期刊>Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis >Analysis of the antimalarial drug halofantrine and its major metabolite N-desbutylhalofantrine in human plasma by high performance liquid chromatography.
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Analysis of the antimalarial drug halofantrine and its major metabolite N-desbutylhalofantrine in human plasma by high performance liquid chromatography.

机译：高效液相色谱法分析人血浆中的抗疟药卤芬太林及其主要代谢物N-去丁基卤氟安汀。

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摘要

The determination of halofantrine and its major metabolite N-desbutylhalofantrine in human plasma by reversed phase high-pressure liquid chromatography is described. The method involves protein precipitation of plasma samples by acetonitrile followed by basification with sodium hydroxide and subsequent liquid-liquid extraction using hexane-diethyl ether (1:1, v/v). The chromatographic separation was carried out on a C-18 column with a mobile phase consisting of methanol/0.05 M KH2PO4 (78:22, v/v) containing 55 mM perchloric acid. Chlorprothixen was used as internal standard. The relative standard deviations of intraday and interday precision for both compounds were less than 7%, the relative standard deviation of the accuracy did not exceed 7.1% at concentrations of 50 and 300 ng/ml. This method is simple, rapid, sensitive and cost effective and was applied to the determination of the pharmacokinetics of halofantrine and N-desbutylhalofantrine in two healthy male volunteers after an oral administrationof 500 mg halofantrine. Moreover, the influence of the frequently consumed kolanut on the pharmacokinetics of halofantrine was investigated.
机译：描述了反相高压液相色谱法测定人血浆中的氟替林及其主要代谢物N-去丁基氟替林。该方法涉及通过乙腈沉淀血浆样品的蛋白质，然后用​​氢氧化钠碱化，然后使用己烷-乙醚（1：1，v / v）进行液-液萃取。色谱分离是在C-18柱上进行的，流动相为包含55 mM高氯酸的甲醇/0.05 M KH2PO4（78：22，v / v）组成。氯噻吨用作内标。两种化合物的日内和日间精度的相对标准偏差均小于7％，在50和300 ng / ml的浓度下，精度的相对标准偏差不超过7.1％。该方法简便，快速，灵敏且具有成本效益，可用于测定口服500 mg氟蒽嘌呤后两名健康男性志愿者中的氟蒽嘌呤和N-去丁基氟蒽嘌呤的药代动力学。此外，还研究了经常食用的古兰经对氟番嘌呤药代动力学的影响。

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