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首页> 外文期刊>Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis >Simultaneous quantification of 14 ginsenosides in Panax ginseng C.A. Meyer (Korean red ginseng) by HPLC-ELSD and its application to quality control.
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Simultaneous quantification of 14 ginsenosides in Panax ginseng C.A. Meyer (Korean red ginseng) by HPLC-ELSD and its application to quality control.

机译：同时定量人参中14种人参皂苷的含量HPLC-ELSD法测定的迈耶（韩国红参）及其在质量控制中的应用。

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摘要

A new method of high-performance liquid chromatography coupled with evaporative light scattering detection (HPLC-ELSD) was developed for the simultaneous quantification of 14 major ginsenosides, which are the marker compounds of Panax ginseng C.A. Meyer (Korean red ginseng). Various types of ginseng samples were extracted, and the amounts of the 14 ginsenosides (Rg1, Re, Rf, Rh1, Rg2, Rb1, Rc, Rb2, Rb3, Rd, Rg3, Rk1, Rg5, and Rh2) were determined by reverse-phase HPLC-ELSD using digoxin as an internal standard. The mobile phase consisted of a programmed gradient of aqueous acetonitrile. Calibration curves for each ginsenoside were determined for the quantification. The method was validated for linearity, precision, accuracy, limit of detection, and limit of quantification. This quantification method was applied to several finished ginseng products including white ginseng, red ginseng powder, and red ginseng concentrate. The amounts of the 14 ginsenosides in the various ginseng samples could be analyzedsimultaneously. This validated HPLC method is expected to provide a new basis for the quality assessment of ginseng products.
机译：开发了一种高效液相色谱结合蒸发光散射检测（HPLC-ELSD）的新方法，可同时定量14种主要人参皂甙，它们是人参C.A的标志物。迈耶（韩国红参）。提取各种类型的人参样品，并通过反向分析确定14种人参皂苷的含量（Rg1，Re，Rf，Rh1，Rg2，Rb1，Rc，Rb2，Rb3，Rd，Rg3，Rk1，Rg5和Rh2）。地高辛作为内标的HPLC-ELSD相。流动相由乙腈水溶液的程序梯度组成。确定每种人参皂苷的校准曲线以进行定量。验证了该方法的线性，精密度，准确性，检测限和定量限。这种定量方法适用于几种人参成品，包括白参，红参粉和红参浓缩物。可以同时分析各种人参样品中14种人参皂苷的含量。这种经过验证的HPLC方法有望为人参产品的质量评估提供新的依据。

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