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首页> 外文期刊>Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis >HPLC determination of four triterpenoids in rat urine after oral administration of total triterpenoids from Ganoderma lucidum.
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HPLC determination of four triterpenoids in rat urine after oral administration of total triterpenoids from Ganoderma lucidum.

机译：口服灵芝中的总三萜类成分后，HPLC测定大鼠尿液中的四种三萜类成分。

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摘要

A sensitive and simple high-performance liquid chromatography (HPLC) method was applied for the quantitative determination of four major triterpenoids (ganoderic acids C(2), B, K and H) in rat urine after oral administration of total triterpenoids from Ganoderma lucidum. The urine sample was extracted with dichloromethane-ethyl acetate (90:10) after acidification by hydrochloric acid (0.2 mol/ml). Chromatographic separation was achieved on a Zorbax SB-C(18) column (250 mm x 4.6 mm, 5 microm) at 35 degrees C, with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/v), at a flow rate of 1.2 ml/min. The four triterpenoids and internal standard (hydrocortisone) were detected at a wavelength 252 nm. The within- and between-day assay coefficients of variation for the four triterpenoids in urine were less than 9% and the extraction recovery of this method was higher than 90%. Using this method, the excretion profile of the triterpenoids in rat urine after oral administration of total triterpenoids of G. lucidum was revealed for the first time.
机译：灵敏和简单的高效液相色谱（HPLC）方法用于定量测定大鼠灵芝中总三萜类化合物口服后大鼠尿液中的四种主要三萜类化合物（灵芝酸C（2），B，K和H）的定量测​​定。用盐酸（0.2 mol / ml）酸化后，用二氯甲烷-乙酸乙酯（90:10）提取尿液样品。在Zorbax SB-C（18）色谱柱（250 mm x 4.6 mm，5 microm）上于35°C，以乙腈和0.03％磷酸水溶液（v / v）线性梯度洗脱进行色谱分离速度为1.2 ml / min。在波长252 nm处检测到了四个三萜类化合物和内标（氢化可的松）。尿液中四种三萜类化合物的日内和日间测定变异系数小于9％，该方法的提取回收率高于90％。使用这种方法，首次揭示了大鼠口服灵芝总三萜类化合物后三萜类化合物的排泄情况。

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