...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of pharmaceutical sciences. >Aggregation of recombinant human botulinum protein antigen serotype C in varying solution conditions: implications of conformational stability for aggregation kinetics.
【24h】

Aggregation of recombinant human botulinum protein antigen serotype C in varying solution conditions: implications of conformational stability for aggregation kinetics.

机译：重组人肉毒杆菌蛋白抗原血清C型在不同溶液条件下的聚集：构象稳定性对聚集动力学的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Solution conditions greatly affect the aggregation rate of a protein. Elucidating these influences provides insight into the critical factors governing aggregation. In this study, recombinant human botulinum protein antigen serotype C [rBoNTC (H(c))] was employed as a model protein. rBoNTC (H(c)) aggregated irreversibly during incubation at 42 degrees C. The aggregation rate was studied as a function of solution conditions, including varying the pH from 3.5 to 8.0 and with or without 150 mM NaCl, 7.5% (w/v) trehalose, and 0.5 M urea. Some solution conditions retarded rBoNTC (H(c)) aggregation, whereas others accelerated aggregation, particularly acidic pH and addition of NaCl or urea. To better understand the mechanism by which these solution conditions influenced aggregation rates, the structure of rBoNTC (H(c)) was characterized using circular dichroism, fluorescence, and ultraviolet absorbance spectroscopies. Conformational stability was assessed from equilibrium urea-induced unfolding studies and by using differential scanning calorimetry (DSC). The activation energy of the aggregation reaction (E(a)) was estimated from an analysis of the heating-rate dependence of the thermal transition observed during DSC heating scans. Overall, for rBoNTC (H(c)), an inverse correlation was found between conformational stability and aggregation rate, as well as between the kinetic barrier to unfolding (i.e., E(a)) and aggregation rate.
机译：溶液条件极大地影响蛋白质的聚集速率。阐明这些影响可以深入了解控制聚合的关键因素。在这项研究中，重组人肉毒杆菌蛋白抗原血清型C [rBoNTC（H（c））]被用作模型蛋白。 rBoNTC（H（c））在42°C的孵育过程中不可逆地聚集。研究了聚集速率与溶液条件的关系，包括pH值从3.5更改为8.0，以及有无150 mM NaCl，7.5％（w / v） ）海藻糖和0.5 M尿素。某些溶液条件会延迟rBoNTC（H（c））聚集，而另一些会加速聚集，特别是酸性pH和NaCl或尿素的添加。为了更好地理解这些溶液条件影响聚集速率的机制，使用圆二色性，荧光和紫外吸收光谱对rBoNTC（H（c））的结构进行了表征。从平衡尿素诱导的展开研究和使用差示扫描量热法（DSC）评估构象稳定性。聚集反应的活化能（E（a））是通过对DSC加热扫描中观察到的热转变的升温速率依赖性进行分析来估计的。总体而言，对于rBoNTC（H（c）），在构象稳定性和聚集速率之间以及在展开的动力学障碍（即E（a））和聚集速率之间发现了反相关关系。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号