...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >NMR investigation of the alkaline-like conformational transition of horse heart cytochrome c in the presence of exogenous thiazole
【24h】

NMR investigation of the alkaline-like conformational transition of horse heart cytochrome c in the presence of exogenous thiazole

机译：外源噻唑存在下马心脏细胞色素c碱性样构象转变的NMR研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The conformational transition of horse heart cyt c in the presence of exogenous thiazole is investigated by NMR spectroscopy. Surprisingly, besides the native form and the ligand-bound form, another species (species A) exists at neutral pH. Titration of thiazole at alkaline condition indicates that this form has Lys as the axial ligands and in fact is one of alkaline cyt c conformers. At a high concentration of thiazole, species A has a dominant population at a pH of approximately 7. It is the first time that a pure alkaline conformer of cyt c is obtained at neutral pH. The observed NOEs show that species A maintains the overall protein fold although large structural rearrangements are expected in the distal pocket. Furthermore, the different effect of imidazole, pyridine and thiazole on the alkaline transformation of cyt c is discussed. (C) 2003 Elsevier Science B.V. All rights reserved. [References: 39]
机译：通过核磁共振波谱研究了在外源噻唑存在下马心脏细胞ct c的构象转变。令人惊讶地，除了天然形式和配体结合形式之外，在中性pH下还存在另一种（物种A）。噻唑在碱性条件下的滴定表明该形式具有Lys作为轴向配体，实际上是碱性Cyt c构象异构体之一。在高浓度的噻唑下，物种A在大约7的pH值下具有显性种群。这是首次在中性pH值下获得纯的Cyt c碱性构象异构体。观察到的NOEs显示，尽管预期在远端囊袋中会发生较大的结构重排，但物种A仍保持了蛋白质的整体折叠。此外，讨论了咪唑，吡啶和噻唑对cyt c碱性转化的不同影响。 （C）2003 Elsevier Science B.V.保留所有权利。 [参考：39]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号