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首页> 外文期刊>Journal of Phytopathology >Comparative molecular analyses of phytoplasmas infecting Sophora japonica cv. golden and Robinia pseudoacacia.
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Comparative molecular analyses of phytoplasmas infecting Sophora japonica cv. golden and Robinia pseudoacacia.

机译：感染日本槐的植原体的比较分子分析。金和刺槐。

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摘要

Phytoplasmas were detected in Sophora japonica cv. golden and Robinia pseudoacacia with diseased branches of witches'-broom collected in Haidian district, Beijing, China. Phytoplasma cells were observed in phloem sieve elements of symptomatic S. japonica cv. golden by transmission electron microscopy. The presence of phytoplasmas was further confirmed by sequence determination of partial gene sequences of 16S rDNA, rp (ribosomal protein) and secY. Phylogenetic trees and virtual restriction fragment length polymorphism (RFLP) analyses indicated that the phytoplasmas causing S. japonica cv. golden witches'-broom (SJGWB) and R. pseudoacacia witches'-broom (RPWB) belong to the 16SrV (elm yellows) group, and they are most closely related to subgroup 16SrV-B, rpV-C and secYV-C jujube witches'-broom (JWB) phytoplasma. Comparative analyses indicated that the phytoplasma of RPWB was closer to the JWB and that R. pseudoacacia might serve as an alternative host plant of JWB phytoplasma.
机译：在日本槐中发现了植原体。采集于北京海淀区的金黄色和刺槐假山毛病病枝。在有症状的日本链球菌的韧皮部筛网元件中观察到了质体细胞。通过透射电子显微镜观察为金黄色。通过对16S rDNA，rp（核糖体蛋白）和secY的部分基因序列进行序列测定，进一步证实了植物原浆的存在。系统发育树和虚拟限制性片段长度多态性（RFLP）分析表明，引起S. japonica cv的植原体。金色女巫的扫帚（SJGWB）和拟金合欢女巫的扫帚（RPWB）属于16SrV（榆黄色）组，它们与16SrV-B，rpV-C和secYV-C枣女巫亚组最相关'-扫帚（JWB）植物原浆。对比分析表明，RPWB的植物原质更接近JWB，而假阿拉伯金合欢可能是JWB植物的替代寄主植物。

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