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首页> 外文期刊>Journal of Phytopathology >Identification of genes for resistance to Bean common mosaic virus and Bean common mosaic necrosis virus in snap bean (Phaseolus vulgaris L.) breeding lines using conventional and molecular methods.
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Identification of genes for resistance to Bean common mosaic virus and Bean common mosaic necrosis virus in snap bean (Phaseolus vulgaris L.) breeding lines using conventional and molecular methods.

机译：使用常规方法和分子方法鉴定对菜豆（Phaseolus vulgaris L.）育种系中的Bean普通花叶病毒和Bean普通花叶坏死病毒具有抗性的基因。

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Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) are among the biggest threats for snap bean production in Bulgaria due to their seed, aphid and mechanical transmission. Old valuable Bulgarian snap bean varieties are being neglected, because of the high percentage of virus-infected seeds. Breeding resistant cultivars is the best way to solve the problem. The genetic control towards both viruses is assured by one dominant I gene and a number of recessive (bc-u, bc-1, bc-12, bc-2, bc-22 and bc-3) genes. Our aim was to identify resistance gene combinations in advanced F8 breeding lines, derived from two crosses (A-8-40-7-2-1 x IVT 7214) and (Zaria x RH 26D), by the application of conventional and molecular approaches. Four methods were applied for the characterization of their resistance gene makeup: (i) leaf-abscission infection test designed to identify I gene by direct inoculation with NL3 strain of BCMNV; (ii) intact-plant infection test with strain NY15 of BCMV to separate immune genotypes, possessing bc-ubc-12, bc-ubc-22, bc-ubc-2bc-3, I, Ibc-12, Ibc-22 or Ibc-3; (iii) PCR analysis with the following markers: SCAR - SW13 (for I gene), SBD5 (for bc-12), ROC11 (for bc-3) and CAPS - eIFE4 (for bc-3); and (4) high-temperature (more than 30 degrees C) infection test with NL3 of BCMNV to provoke systemic necrosis in I, Ibc-1, Ibc-12, Ibc-12bc-22 or Ibc-3. The four methods applied worked properly and complemented each other. Valuable gene combination (Ibc-3) was established in seven breeding lines with immune reaction to BCMNV. They will be included in the snap bean breeding programme for virus resistance.
机译：豆类普通花叶病毒（BCMV）和豆类普通花叶坏死病毒（BCMNV）由于其种子，蚜虫和机械传播而成为保加利亚速食豆生产的最大威胁。由于有价值的病毒感染种子，保加利亚珍贵的老鹰嘴豆品种被忽视了。育种抗性品种是解决这一问题的最佳方法。一个显性I基因和一些隐性基因（bc-u，bc-1，bc-1 2 ，bc-2，bc-2 2 和bc-3）基因。我们的目的是通过应用常规方法和分子方法，鉴定源自两个杂交（A-8-40-7-2-1 x IVT 7214）和（Zaria x RH 26D）的高级F8育种系中的抗性基因组合。四种方法用于表征其抗性基因组成：（i）脱落叶感染试验，旨在通过直接接种BCMNV的NL3株来鉴定I基因; （ii）用BCMV NY15株进行完整植物感染试验，分离出具有bc-ubc-1 2 ，bc-ubc-2 2 ，bc-ubc的免疫基因型-2bc-3，I，Ibc-1 2 ，Ibc-2 2 或Ibc-3; （iii）具有以下标记的PCR分析：SCAR-SW13（用于I基因），SBD5（用于bc-1 2 ），ROC11（用于bc-3）和CAPS-eIFE4（用于bc- 3）; （4）用BCMNV的NL3进行高温（超过30摄氏度）感染试验，以引起I，Ibc-1，Ibc-1 2 ，Ibc-1 2的系统性坏死 bc-2 2 或Ibc-3。所应用的四种方法工作正常且相辅相成。在七个对BCMNV有免疫反应的育种系中建立了有价值的基因组合（Ibc-3）。它们将被包括在鹰嘴豆育种计划中以提高抗病毒能力。

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